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《Journal of Plant Resources and Environment》 2009-02
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Effects of Pb stress on seed germination and root-tip cell mitosis of Iris lactea var.chinensis

WANG Hong-yan,HUANG Su-zhen①(Institute of Botany,Jiangsu Province and the Chinese Academy of Sciences,Nanjing 210014,China)  
The effects of Pb stress with different concentrations on seed germination and root-tip cell mitosis of Iris lactea Pall.var.chinensis(Fisch.) Koidz.were studied by water culture method.The results show that within 500-1 500 mg·L-1 Pb,the seed germination energy,seed germination rate,seed germination index,seed vigor index and root-tip cell mitosis index of seedling of I.lactea var.chinensis decrease gradually with the increase of Pb concentration.Under 500,1 000 and 1 500 mg·L-1 Pb stresscondition,the seedgerminationenergyof I.lacteavar.chinensis is 52.8%,51.3% and 47.2% of that of the control.The seed germination rate is 55.8%,54.3% and 46.5% of that of the control.The seed germination index decreases 55.1%,56.7% and 57.8% compared to the control.The seed vigor index decreases 65.4%,72.0% and 79.2% compared to the control.And the root-tip cell mitosis index is only 28.1%,21.9% and 13.5% of that of the control.There are significant differences between the indexes of treatment groups and the control(P0.05),but the indexes among treatment groups are not significantly different except the seed vigor index.It can be concluded that the seed germination and root-tip cell mitosis of I.lactea var.chinensis are inhibited obviously under Pb stress,but they are not sensitive to the stress when the concentration of Pb is higher than 500 mg·L-1.
【Fund】: 国家自然科学基金资助项目(30771520)
【CateGory Index】: X173
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