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《Chinese Journal of Reparative and Reconstructive Surgery》 1993-04
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EXPERIMENTAL STUDIES OF EFFECTS OF CHITOSAN ON THE GROWTH OF HUMAN CELLS IN VITRO

Sheng Zhijian, Hou Cunlin. In- stitute of Traumatic Surgery, Chang Zheng Hospital, Shanghai, 200003  
Human fibroblasts and human epidermal keratinocytes were used for culture. Chitosan solution were added in the culture solution(DMEM). After 72 hours, the fibroblasts showed rapid growth in the control culture without Chitosan, But the numbers of human fibroblasts from growth was decreased as the concentration of Chitosan was increasing. On the contrary the human epidermal keratinocytes growed more rapidly in the culture with Chitosan than in the culture without Chitosan. The results showed that Chitosan inhibited the growth of human fibroblast and stimulated the growth of human epidermal keratinocyte.
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