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《China Journal of Modern Medicine》 2010-16
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Effects of sevoflurane alleviating LPS-induced acute lung injury by increasing HO-1 expression

ZHAO Shuang-ping,WU Jiao,GUO Qu-lian,CHEN Sheng-xi(ICU,Xiangya Hospital,Central South University,Changsha,Hunan 410008,P.R.China)  
【Objective】 To investigate the effect of heme oxygenase-1 in sevoflurane inhalation lessening acute lung injury induced by lipopolysaccharide(LPS) in rats.【Methods】Thirty-six male SD rats weighing 220~250 g were randomly divided into 6 groups(n =6 each):group Ⅰcontrol(group C),group ⅡZnPP,group Ⅲ sevoflurane(group Sev),group Ⅳ LPS-Sev,group Ⅴ ZnPP-LPS-Sev and group Ⅵ LPS.In group Ⅱ and Ⅴ,ZnPP 10 mg/kg was given.10 min later,in group Ⅰ,Ⅱ and Ⅵ,normal saline and LPS 5 mg/kg were given.Sevoflurane(end-tidal concentration 2.4%) were inhaled in group Ⅳ and Ⅴ for 4 h after iv injection of LPS 5 mg/kg respectively.Sevoflurane was inhaled in group Ⅲfor 4 h after iv injection of normal saline.The animals were killed at 6 h after LPS or normal saline administration.Lungs were removed for determination of W/D lung weight ratio,myeloperoxidase(MPO) activity,MDA content,SOD activity and expression of HO-1 and HO-1 mRNA.The severity of lung injury was evaluated using lung histopathology score.【Results】 Injection of LPS increased W/D lung weight ratio,MPO activity,MDA content,lung histopathology score and HO-1 expression,and decreased SOD activity.Inhalation of sevoflurane decreased W/D lung weight ratio,MPO activity,MDA content and lung histopathology score,increased SOD activity and HO-1 expression.The beneficial effects of sevoflurane were abolished by ZnPP.【Conclusion】 Sevoflurane could protect the lungs against LPS-induced acute lung injury by increasing HO-1 expression.
【Fund】: 湖南省科技厅发展基金项目(No:06SK3622)
【CateGory Index】: R563.8
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