In vitro effects of reduning on CBV3 and ECHO11
DONG Gui-yan1,CHEN Zong-bo2,XIAO Wei3,YANG Jin3,CHEN Feng2, ZHAO Bao-chun2,ZHANG Ping2(Department of Pediatrics,the Affiliated Hiser Hospital,Medical College University of Qingdao Qingdao,Shandong 266033,P.R.China)
【Objective】 To study the anti-enterovirus effect of reduning Inject in-vitro.【Methods】 Adopting the technique of cell culture to observe cytopathogenic effect(CPE) and MTT colorimetry,plaque-reduction assay to evaluate the anti-enterovirus effect of Reduning,Yanhuning and Ribavirin.The anti-enterovirus effect of Reduning and Yanhuning were also compared before and after ECHO 11 absorbing cytopathic plaque-reduction assay.【Results】 ①The TC50 of reduning,Yanhuning and ribavirin was 52.0 mg/mL,2.0 mg/mL,2.0 mg/mL respectively.Reduning could inhibit CBV3 and ECHO11 at the concentration of 1.3 mg/mL and the viral inhibiting effect was concentration-dependent.Yanhuning,ribavirin could inhibit CBV3 and ECHO11 at the concentration from 0.4 mg/mL to 1.2 mg/mL and from 0.4 mg/mL to 1.2 mg/mL respectively.②The plaque-reduction rate of 26.0 mg/mL reduning was 79.8% and 88.6%,of 0.8 mg/mL Yanhuning was 67.0% and 76.6%,of 1.5 mg/mL Ribavirin was 44.2% and 41.9% respectively.③The anti-ECHO11 activity of Reduning before ECHO11 absorbing was higher than after ECHO11 absorbing(P 0.05),there was no significant difference between the plaque-reduction rates of Yanhuning before and after ECHO11 absorbing(P 0.05).【Conclusion】 The three drugs all have the anti-virus activity in vitro while reduning are most effective.Among these three drugs,the cytotoxicity of reduning is most weak.The anti-virus activity of Reduning adding before virus absorbing was higher,so it seems that reduning can prevent EV infection.
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