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《中国结合医学杂志(英文版)》 2015-08
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The Expression of Calcitonin Gene-related Peptide on the Neurons Associated Zusanli(ST 36) in Rats

CUI Jing-jing;ZHU Xin-long;SHI Hong;SU Yang-shuai;JING Xiang-hong;BAI Wan-zhu;Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences;  
Objective:To investigate the biochemical characteristic of the neurons associated Zusanli(ST 36) in the rat by using Alexa Fluor 594 conjugated cholera toxin subunit B(AF594-CTB) neural tracing and calcitonin gene-related peptide(CGRP) fluorescent immunohistochemical techniques.Methods:Four male Sprague Dawley rats were injected with AF594-CTB into the corresponding area of the Zusanli in the human body.After 3 surviving days,the rat's spinal cord and dorsal root ganglia(DRGs) at lumbar segments were dissected following perfusion with 4%paraformaldehyde,cut into sections,and then stained with CGRPfluorescent immunohistochemical method.Results:AF594-CTB labeled sensory neurons were detected in the L3-L6 DRGs with high concentration in L4 DRG,and the labeled motor neurons located in the dorsolateral and intermediate regions of lamina Ⅸ from L3-L5 segments with high concentration at L4.Meanwhile,CGRPpositive neural labeling distributed symmetrically on both sides of DRGs,anterior and dorsal horns of spinal cord.In the AF594-CTB labeled neurons,37%sensory neurons and 100%motor neurons expressed CGRPpositive.Conclusion:These findings present the morphological evidence to demonstrate that the sensory and motor neurons associated Zusanli in the rat distributed with segmental and regional patterns,and contained CGRP-expression.
【Fund】: Supported by the National Natural Science Foundation of China(No.81072759);; the National Basic Research Program of China(973 program No.2011 CB505201);; the Self-selected Research Program from China Academy of Chinese Medical Sciences(No.2009Z02024)
【CateGory Index】: R245
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