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《Chinese Journal of New Drugs》 2009-09
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Determination of aesculin in Huanxiangtong eye drops by HPLC

FAN Xiao-ling1,GUO Song2(1 Hospital of China University of Petroleum East China,Dongying 257061,China;2 Beijing Boyakangtai Bioenginetech Co.,Ltd,Beijing 100083,China)  
Objective: To establish an HPLC method for determining aesculin in Huanxiangtong eye drops.Methods: The samples were analyzed by HPLC using a YMC C18 column(250mm×4.6mm,10μm),the mobile phase was consisted of acetonitrile-0.1% phosphoric acid(12∶88) at the flow rate of 1.0mL·min-1,the detection wave-length was at 334nm,and the column temperature was 30℃.Results: A linear range of aesculin curve was within 0.0284~0.2840μg(r=0.9996),the average recovery was 99.16% and the RSD was 0.89%.The content of aesculin was 1.51mg per bottle.Conclusion: The method is simple,reproducible and accurate,therefore it can be used for the quality control of Huanxiangtong eye drops.
【Fund】: 国家科技部中小企业创新基金(05C26211100134)
【CateGory Index】: R286.0
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