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《Journal of Chinese Medicinal Materials》 2006-12
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Screening the Active Components of Salvia miltiorrhiza for Atherosclerosis with Chromatography of CD40 Highly Expressed Cell Membrane

LIN Rong(1,2),YANG Guang-de1,WANG Wei-rong1,LIU Jun-tian1,HE Lang-chong1,PENG Ning1,HAN Chun-jie1,LIU Yu1(1.Xi'an Jiaotong University,School of Medicine,Xi'an 710061,China;2.Xi'an Jiaotong University,Key Laboratory of Environment and Genes Related to Diseases,Xi'an 710061,China)  
Objective:To screen the effective components of Salvia miltiorrhiza for atherosclerosis with chromatography of CD40 highly expressed cell membrane.Methods: The stable high expression of CD40 in human endothelial cells ECV-304 was constructed,and the cell membrane stationary phase(CMSP) was prepared to screen the effective anti-atherosclerostic components of Salvia miltiorrhiza with it.Results: It was found that liposoluble and water soluble extracting site from Salvia miltiorrhiza had affinity for the cell membrane with highly expressed CD40,in which Tanshinone ⅡA、 Tanshinone ⅠA and Danshensu were effective components.Conclusion: The model of the newly constructed cell membrane chromatography may be used to screen drugs for atherosclerosis.
【Fund】: 国家自然科学基金资助(No.30371759)
【CateGory Index】: R285
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