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《Journal of Chinese Medicinal Materials》 2007-10
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Effect of Luteolin on COX-2 and mPGES-1 Expression in LPS-induced RAW264.7 Cells

WANG Xu-guang,CHEN Gen-yin,CHEN Miao-ping(First Affiliated Hospital of Guangdong Medical College,Zhanjiang 524100,China)  
Objective: To study the effect of luteolin on COX-2 and mPGES-1 expression in LPS-induced RAW264.7 cells.Methods: After being pretreated with different concentrations of luteolin for 30 min,and then incubated with 1 μg/ml LPS for 12h,the effect of luteolin on the product of PGE2 in RAW264.7 cells was measured by enzyme immunoassay(EIA).The mRNA expression of COX-2 and mPGES-1 in RAW264.7 cells were analysed by RT-PCR.The COX-2 and mPGES-1 protein expression in RAW264.7 cells were analysed by western blotting.Results: Luteolin inhibited the LPS-induced PGE2 synthesis in RAW264.7 cells.The mRNA and protein expression of COX-2 and mPGES-1 in RAW264.7 cells were also decreased by luteolin.Conclusion: Luteolin can inhibit significantly the expression of COX-2 and mPGES-1 in PGE2 synthetic pathway.
【Fund】: 湛江市科技攻关项目(编号:2004090);; 广东医学院青年基金项目(编号:2004109)
【CateGory Index】: R285.5
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