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《Journal of Chinese Medicinal Materials》 2008-08
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Effect of Total Flavones from Cuscuta chinensis on Expression of Th Type-1/Th Type-2 Cytokines,Serum P and PR in Abortion Rats Model

MA Hong-Xia1,YOU Zhao-ling2,WANG Ruo-guang2(1.The First Affiliated Hospital of Guangzhou Medicine college,Guangzhou 510405,China;2.Hunan Traditional Chinese Medicine University,Changsha 410208,China)  
Objective: To study the effect of TFCC on expression of Th type-1/Th type-2 cytokines and PR in rats abortion model,and observe the changes of serum P and PRL.Methods: Early abortion rat model was established by Bromocriptine at 68 gestation day,the model rats were treated with high and low dosage of TFCC and the progesterone as the positive control.Progesterone and prolactin levels in the serum were measured by radioimmunoassay,the protein expression of PR was inspected by immunohistochemical,RT-PCR was applied to determine the expression of Th1/Th2 mRNA on deciduas.Results: In the deciduas of model group,IL-4 and IL-10 cytokine contens were clearly lower than that of normal pregnancy group.The difference was notable(P0.05),In the deciduas of model group,IFN-γ and TNFα cytokine contents were clearly higher than that of normal pregnancy group.The difference was notable(P0.05),TFCC could up-regulate the level of IL-4 and IL10,and down-regulate the level of TNF-α and IFN-γ,which could promote P secretion and up regulate PR mRNA expression on deciduas.Conclusion: TFCC can regulate endocrinologycal and immunological network equilibration on maternal-fetal face,finally achieve prevention abortion.
【Fund】: 广东省自然科学基金(5300997);; 广州市卫生局资助项目(2005A041)
【CateGory Index】: R285.5
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