Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Chinese Medicinal Materials》 2009-12
Add to Favorite Get Latest Update

Extraction,Selenium-nanoparticle Preparation and Anti-virus Bioactivity Determination of Polysaccharides from Caulerpa taxifolia

MEN Xiao-yuan,XU Wen-gui,ZHU Xiang,MA Wen-chao (Department of PET-CT,Tianjin cancer hospital & institute,Tianjin Medical University/Key Laboratory of Cancer Prevention and Therapy,Tianjin 300060,China)  
Objective:To extract polysaccharides from Caulerpa taxifolia and determine its anti-virus bioactivity.Methods:The crude polysaccharides were extracted by hot water and precipitated with ethanol,then they were further purified by DEAE-Cellulose and Sephadex G-200 chromatography.After determined by chemicophysical analysis,the polished polysaccharides were selenium treated and bio-assayed by MTT and CPE methods.Results:The crude extraction from Caulerpa taxifolia was a kind of sulfated polysaccharides in which the average content of polysaccharides and sulfate were 27.9%and 11.5%,respectively.The recovery rate of the polished polysaccharides was 66.3%after the 2-column purification.The IC_(50) and TI of the purified polysaccharide SCp11 were 2.2 mg/ mL and 3057.0,respectively.After treated with selenium,the average diameter of the nanoparticle was 28.6 nm,and its bioactivity and TI index were enhanced significantly although with a higher cytotoxicity.Conclusion:The polysaccharides from Caulerpa taxifolia and its selenium particles was a kind of bioactive substance for anti-virus drug-candidate development.
【Fund】: 国家自然科学基金(30872954);; 天津市自然科学基金重点项目(08JCZDJC23700);; 天津市科委面上项目(07JCYBJC09300)
【CateGory Index】: R284;R285
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 2 Hits
1 GUO Xin1, GAO Xiang-dong1, YANG Xiao-bing2(1.School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China;2. School of Life Sciences, Nanjing University, Nanjing 210093, China);Determination of content glucuronic acid and neutral sugar of acidic polysaccharide[J];Chinese Journal of Biochemical Pharmaceutics;2004-02
2 Chen Fuxiang; Liu Jingxing; Lu Deyuan(Department of Microbiology, Shanghai Second Medical University, 200025);The Effects of Sophora Flavescens Alkaloids (SFA) on Coxsackie Virus B3(CVB3)in Vitro and a Preliminary Study of Its Mechanism[J];CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY;1995-02
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 SHANG Peng 1, YANG Tie Hong 2, JIA Min 2,MEI Qi Bing 2, ZHAO Wen Ming 1, CAO Zhi Xian 3, ZHAO De Hua 2 1Institute of Bioengineering, Xi'an Jiaotong University, Xi'an 710049, 2Department of Pharmacology, Faculty of Preclinical Med;Purification and analysis of polysaccharides of Angelica sinensisi (Oliv.) Diels[J];Journal of The Fourth Military Medical University;2001-14
2 Zhang Xiaoling (Basic Courses Department, Gansu Agricultural University, Lanzhou 730070);Spectrophotometric Method for the Determination of Pectin with Carbazole and Sulphuric Acid[J];JOURNAL OF GANSU AGRICULTURAL UNIVERSITY;1999-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved