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《Journal of Chinese Medicinal Materials》 2009-12
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Extraction,Selenium-nanoparticle Preparation and Anti-virus Bioactivity Determination of Polysaccharides from Caulerpa taxifolia

MEN Xiao-yuan,XU Wen-gui,ZHU Xiang,MA Wen-chao (Department of PET-CT,Tianjin cancer hospital & institute,Tianjin Medical University/Key Laboratory of Cancer Prevention and Therapy,Tianjin 300060,China)  
Objective:To extract polysaccharides from Caulerpa taxifolia and determine its anti-virus bioactivity.Methods:The crude polysaccharides were extracted by hot water and precipitated with ethanol,then they were further purified by DEAE-Cellulose and Sephadex G-200 chromatography.After determined by chemicophysical analysis,the polished polysaccharides were selenium treated and bio-assayed by MTT and CPE methods.Results:The crude extraction from Caulerpa taxifolia was a kind of sulfated polysaccharides in which the average content of polysaccharides and sulfate were 27.9%and 11.5%,respectively.The recovery rate of the polished polysaccharides was 66.3%after the 2-column purification.The IC_(50) and TI of the purified polysaccharide SCp11 were 2.2 mg/ mL and 3057.0,respectively.After treated with selenium,the average diameter of the nanoparticle was 28.6 nm,and its bioactivity and TI index were enhanced significantly although with a higher cytotoxicity.Conclusion:The polysaccharides from Caulerpa taxifolia and its selenium particles was a kind of bioactive substance for anti-virus drug-candidate development.
【Fund】: 国家自然科学基金(30872954);; 天津市自然科学基金重点项目(08JCZDJC23700);; 天津市科委面上项目(07JCYBJC09300)
【CateGory Index】: R284;R285
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