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《Chinese Archives of Traditional Chinese Medicine》 2010-01
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Determination of Tannins and Chlorogenic Acid of Different Vegetative Organs in Emilia sonchifolia(Linn.) DC

ZHANG Yan,ZHANG Hong-bin,CHEN Guang-zhou,WANG Yan(Department of Life and Science,Qiongzhou College,Wuzhishan 572200,Hainan,China)  
Objective:To establish the analytical methods for determinating the contents of tannins and chlorogenic acid of different vegetative organs in Emilia sonchifolia(Linn.) DC.Methods:The content of tannins,expressed as gallic acid equivalents,was measured by using the phospho molybdenum tungstic acid-casein colorimetry,and the content of chlorogenic acid,expressed as chlorogenic acid equivalents,was measured by HPLC.Results:The good linearity between concentrations and absorbances of gallic acid was found in the range of 2 ~ 10 mg L-1,the average recovery was 100.32% and RSD was 1.45% ,the contents of tannins in leaves,stems and roots of Emilia sonchifolia(Linn.) DC were 0.25% ,0.15% and 0.22% respectively;The good linearity between content and peak area integral value of chlorogenic acid was found in the range of 0.0356 ~ 0.5340μg,the average recovery was 99.37% and RSD was 1.52% ,the contents of chlorogenic acid in leaves,stems and roots of Emilia sonchifolia(Linn.) DC were 1.28% ,1.14% and 0.91% respectively.Conclusion:Two proposed methods are sensitive,precise,accurate and reproducible,and they are suitable for the determination of tannins and chlorogenic acid in Emilia sonchifolia(Linn.) DC.
【Fund】: 海南省教育厅高等学校科研资助项目(Hjkj2009-59)
【CateGory Index】: R284.1
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Chinese Journal Full-text Database 3 Hits
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