Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Archives of Traditional Chinese Medicine》 2013-04
Add to Favorite Get Latest Update

Studies on Separation and Purification of Total Flavones from Gynostemma Pentaphyllum with Macroreticular Resin

YANG Xiuzhen(Jiangsu Provincial Hospital of Traditional Chinese Medicine,Nanjing 210029,Jiangsu,China)  
Objective:To set up the technology for adsorbing and separating total flavone in Gynostemma pentaphyllum with macroporous adsorption resin,and D101 macroporous adsorption resin was invested.Methods:The static adsorption and separation were used in investigation of macroporous adsorption and separation;the dynamic adsorption and adsorption were used in studying the condition of adsorption and separation.The total flavone adsorption capacity and desorption rate were used as the evaluation criteria.The UV spectrophotometric method was used in the determination of total flavone content.Results:The results showed that the D101 was the best for adsorbing and separating the total flavone in Gynostemma pentaphyllum in the following technological conditions:the concentration of Gynostemma pentaphyllum sample extract was 8.95 mg/mL;the maximum adsorbing capacity for total flavone in Gynostemma pentaphyllum was 9.06 mg/mL;the current velocity was 6BV/h;the eluting reagent was 50% ethanol.Conclusion:It is the best way to use D101 resin to separate the total flavone in Gynostemma pentaphyllum under the technological conditions,the purification technology is better,the method of purifying total flavones is feasiable.
【CateGory Index】: TQ461;R284.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 4 Hits
1 YAN Yan,GUO Jie,YANG Fei,XUE Wei(Shangdong Provincial Centre for Disease Control and Prevention,Jinan 250014,China);Anti-Oxidative Effect of Gynostemma and Hawthorn Extracts on the Aging Mouse Model Induced by D-galactose[J];Laboratory Animal Science;2009-03
2 SHEN Hong-wei1,XIAO Yan-chun2,CHE Ren-guo1,JIN Feng-xie1,YU Hong-shan1(1.School of Food Science & Biotechnology,Dalian Polytechnic University,Dalian,116034,China;2.Agricultural Product Quantity Analysis Center,Liaoning Agricultural Vocation-technical College,Yingkou,115009,China);Research Progress on the Chemical Ingredients of Gynostemma pentaphyllum[J];Lishizhen Medicine and Materia Medica Research;2008-07
3 LI-le,SUN Xiao-dong,GAO Xiao-li,DING Bao-xing,YUAN Bin-xiang;Protective effects of total flaveos of Gynostemma pentaphyllum(Thunb) Mak against acute myocardial ischemia in anesthetized dogs[J];Chinese Journal of Pathophysiology;2008-02
4 LI Le School of Pharmacy,Zhejiang University of Technology,Hangzhou 310014,China; GAO Xiao-li DING Bao-xing YUAN Bin-xiang Department of Pharmacology,Medical College,Xi'an Jiaotong University,Xi'an 710061,China;Effect of total flaveos of Gymostemma pentaphyllum on protein expression of Fas/FasL genes and TNF-α concentration in cultured neonatal rat cardiomyocytes with hypoxia-reoxygenation[J];China Journal of Chinese Materia Medica;2007-18
Chinese Journal Full-text Database 10 Hits
1 RUAN Hong-sheng et al(Life Science and Technology College,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319);Preparation of Solid Beverage of Effervescent Tablet from Radix isatidis[J];Journal of Anhui Agricultural Sciences;2010-07
2 YANG Qiang1,XIE Hui-chen1,PAN Qing-hua2,LIU Xiao-qin3,XU Jian-hua1, ZHAO Xiao-qin1,JIANG Jin-lin1(1.Department of Traditional Chinese Medicine,Affiliated Hospital,Hubei University for Nationalities, Enshi 445000,China;2.Department of Digestive Medicine,Central Hospital,Enshi Autonomous Prefecture, Enshi 445000,China;3.Department of Preventive Medicine,Center for Disease Control and Prevention of Enshi,Enshi Autonomous Prefecture,Enshi 445000,China);Evaluation on curative effect of Self-prepared Softening Liver and Eliminating Fat Decoction in treatment of non-alcoholic steatohepatitis[J];Journal of Jilin University(Medicine Edition);2012-01
3 LIU Hai,YANG Yu,JIN Feng-xie,YU Hong-shan(School of Biological & Food Engineering,Dalian Polytechnic University,Dalian 116034,China);Isolation and identification of gypenoside in gypenoside hydrolysate[J];Journal of Dalian Polytechnic University;2010-02
4 MAO Ze-ren1,ZHANG Jing2,JIN Feng-xie1,YU Hong-shan1 1.School of Biological Engineering,Dalian Polytechnical University,Dalian 116034,China; 2.Dalian Institute of Product Quality Supervision Inspection,Dalian 116021,China;Purification and characterization of gypenosidase[J];Journal of Dalian Polytechnic University;2012-02
5 PAN Zhi-hao1,YE Zhen-zhen2,CUI Sheng-miao2(1.Chencun Hospital of Shunde,Foshan 528313,China;2.School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China);Study on extraction process of total flavonoids from Gynostemma pentaphyllum[J];Journal of Guangdong Pharmaceutical University;2012-01
6 TANG Yan-qing1,QIU Dan-ping1,LUO Yong-lin1,HUANG Suo-yi2(1.Undergraduate of Grade 2006,Department of Clinical Laboratory Medicine,Youjiang Medical College for Nationalities,Baise 533000,China;2.Teaching and Research Section of Chemistry,Department of Pharmacy,Youjiang Medical College for Nationalities,Baise 533000,China);Optimization of Extraction Technology for Total Saponin in Mango Leaf[J];Technology & Development of Chemical Industry;2009-07
7 LUO Yong-lin1,QIU Dan-ping1,TANG Yan-qing1,HUANG Suo-yi2,LU Hai-feng2(1.Department of Laboratory Medicine,Youjiang Medical College for Nationalities,Baise 533000,China;2.Teaching and Research Section of Chemistry,Department of Pharmacy,Youjiang Medical College for Nationalities,Baise 533000,China);Optimization of Extraction Conditions of Total Saponins from Argyreia Acuta Lour[J];Technology & Development of Chemical Industry;2010-01
8 Zhou Juan Luo Yu Wu Yaosheng Li Kezhi Jiang Junfu Xu Peng Dept. of Biochemistry and Molecular Biology,Guangxi Medical University,Nanning,530021;RAPD Primers Screening and Genetic Diversity Analysis of Eight Germplasms of Gynostemma Pentaphyllum from Guangxi[J];Genomics and Applied Biology;2010-02
9 LIANG Yan1,2,XU Xiang-wei1,ZHAO Zhuan-you1 1.Tianjin Centre for New Drug Safety Assessment and Research,Tianjin 300193,China 2.Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;Protection effect of Xiangqinglan Dripping Pill on acute myocardium ischemia in rats induced by coronary artery ligation[J];Drugs & Clinic;2013-03
10 LUO Lan(Department of pharmacy,Fujian health college Fuzhou 350101,China);Application of membrane separation technology in chinese medicine industry[J];Strait Pharmaceutical Journal;2011-10
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Xia,WANG Ping-jun,Xu Fu-xin (The Frist People's Hospital of Wuhu,Anhui Wuhu 241000,China);STUDY ON GYPENOSIDES INHIBITING NEOPLASM GROWTH AND ELEVATING IMMUNOLOGICAL FUNCTION IN LEWIS LUNG CANCER OF MICE[J];Journal of Anhui Traditional Chinese Medical College;2001-01
2 GE Jun,ZHU Yu-jie,WU Jun,YAO Bei-bei (Faculty of Clinical Medicine,Bengbu Medical College,Bengbu 233030,China);Protective effects of gypenosides on the heart injury after intestinal ischemia-reperfusion in rabbits[J];Journal of Bengbu Medical College;2006-04
3 WAN Li, WAN Xing-Wang, HU Jin-Hong (1. Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2. International Cooperation Laboratory of Signal Transduction, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438);Effect of gypenosides on hepatic function and fibrosis in rats with immune fibrosis[J];Academic Journal of Second Military Medical University;2003-12
4 CHENG He Xiang 1, ZHANG Rong Qing 1, WANG Hai Chang 1, LI Fei 1, JIA Guo Liang 1, ZHAO Xin Guo 1, CHEN Dan 2, LIU Bing 1 1Department of Cardiovasology, Xijing Hospital, 2Center of Electron Microscope, Faculty of Preclinical Medicine, Fourth Military Medical University, Xian 710033, China;Effects of taurine on intracellular free calcium concentration of cultured neonatal rat cardiomyocytes during hypo-xia/reoxygenation[J];Journal of The Fourth Military Medical University;2003-03
5 HOU Hui-li,FU Tong-sheng(College of Animal Medicine,Hunan Agricultural University,Changsha,Hunan,410128,China);Research Advance in the Chemical Ingredients and Pharmacological Effects of Gynostemma pentaphyllum[J];Progress in Veterinary Medicine;2006-S1
6 Zhang Ruizhe,Traditional Chinese Medicine Related Company,Heilongjiang(150056);Research Progress in Pharmacology and Clinical Of Gynostemma Pentaphyllum(Thunb)Maxcino[J];Heilongjiang Medical Journal;2000-05
7 TANG Xiao-qing,ZHU Bing-yang,CHEN Jian-xiong,et al (Institute of Pharmacy & Pharmacology,Nanhua University,Hengyang,Hunan 421001,China) [WT5HZ];Protective Effect of Gypenosides on Ischemic Brain Damage Induced by Photochemistry[J];Journal of Hengyang Medical College;2001-02
8 ZHANG Yong,DING Guohua,ZHANG Jiane,et alDivision of Nephrology,The Affiliated Renmin Hospital of Wuhan University,Wuhan (430060);Effect of Gypenosides on Expression of Connective Tissue Growth Factor(CTGF) in UUO Rats with Renal Tubulointerstitial Fibrosis[J];Chinese Journal of Integrated Traditional and Western Nephrology;2005-07
9 DUAN Bing-Nan,CHENG Qin-lin(Department of Histology and Embryology of XiangYa Medical College of Central South University,changsha 410013,China);Effects of Gypenosides on Enzyme Activity and Phagocytic Capacity of Peritoneal Macrophage in Mice[J];Acta Academiae Medicinae Jiangxi;2007-03
10 WANG Qing-hao,ZHANG Xiong-lu(Department of Chemistry and Life Science,Gannan Teachers' College,Ganzhou 341000,China);STUDY ON REFINE TECHNICS OF FLAVONES FROM GYNOSTEMMA PENTEPHYLLUM WITH MACRORETICUL ARADSORBENT[J];Journal of Chemical Industry of Forest Products;2005-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved