Effect of Plant Growth Substance on Growth of Dendrobium officinale Kimura et Migo
DAI Zhonghua;ZHU Hua;DA Fangfang;ZHANG Miao;LONG Li;Chengdu University of TCM;Guangxi University for Nationalities;Guangxi University of Chinese Medicine;
Objective: To investigate the effect of plant growth substance on the growth and development of Dendrobium officinale Kimura et Migo. Results: Plant growth regulator plays a very important role in the process of tissue culture rapid propagation of Dendrobium officinale. The main control methods as follows: 1To promote the growth of Dendrobium officinale by adjusting the scale of endogenous hormones. 2Regulate chlorophyll content and sucrose metabolism related enzymes to influence the polysaccharide content accumulation of Dendrobium officinale. 3Plant growth regulators can improve the activity of antioxidant enzymes to increase the stress resistance of Dendrobium officinale. Conclusion: It is a practical way to study the changes rule of the effective components content of Dendrobium officinale by using molecular biology technique and combining with the changes of plant hormones of Dendrobium officinale,plant hormone synthesis key enzymes and gene expression analysis.