Effects of Resveratrol on Proliferation and Differentiation of Preadipocytes and Regulation of Related Signaling Pathways
ZHANG Hui;ZHENG Li;BAO Yi;Department of Pharmacy,Jiaxing Second Hospital;Central Laboratory,Jiaxing Second Hospital;
Objective: To investigate the effect of resveratrol on the proliferation and differentiation of mice 3 T3-L1 preadipocytes and the regulation of related signaling pathways. Methods: Mice 3 T3-L1 preadipocytes were cultured and treated with different doses of resveratrol for 24 h and 48 h. Cell proliferation was detected by MTT assay. In the process of induction of 3 T3-L1 preadipocyte differentiation,100 μmol/L resveratrol was treated for 48 h,and the degree of differentiation of adipocytes was observed by oil red O staining after 6 days of continuous culture. PPARγ,CCAAT/enhancer binding protein-α( C/EBPα) and Notch1,inflammation-related gene monocyte chemotactic factor( MCP-1),tumor necrosis factor-α( TNF-α) and leptin( leptin) were detected by fluorescence quantitative polymerase chain reaction( RT-PCR). Enzyme-linked immunosorbent assay( ELISA) was used to analyze the effect of resveratrol on MCP-1 protein secretion during 3 T3-L1 differentiation. Results: Resveratrol significantly inhibited the proliferation of 3 T3-L1 preadipocytes and showed a dose-dependent effect. Compared with the control group,the resveratrol inhibited the synthesis of lipid droplets during the differentiation of 3 T3-L1 cells by inhibiting the expressions of PPARγ,C/EBPα and Notch1 mRNAs in adipocytes. The expression of Leptin mRNA in mature adipocytes induced by white reedosterol was decreased on the second day,and the expression of Leptin mRNA in the adipocytes of adipocytes induced by albinool was significantly lower than that of the control group( P 0. 05) but increased on day 4. Conclusion: Resveratrol can inhibit the proliferation of 3 T3-L1 preadipocytes and preadipocyte differentiation. The mechanism may be through inhibition of PPARγ,C/EBPα and Notch1 expression. In the process of adipocyte differentiation of 3 T3-L1,resveratrol can promote the expression and secretion of MCP-1 during adipocyte differentiation and increase the expression of Leptin gene and regulate the effect of inflammation on obesity.
【Fund】： 国家自然科学基金青年科学基金项目(81101707);; 浙江省自然科学基金项目(LY16H070007);; 浙江省中医药科技计划项目(2014ZB119);; 嘉兴市科技计划项目(2016BY28022)
【CateGory Index】： R285
【CateGory Index】： R285