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《Chinese Wild Plant Resources》 2010-06
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Study on the Extraction Process of Total Amino Acids from Lobaria kurokavae Yoshim

Wang Xiaomei1,2,Zhao Weixing1,2,Chang Junlin3,Liu Sha1,Fang Mi1(1.Department of Chemistry and Chemical Engineering,Baoji University of Arts and Sciences,Baoji 721013,China;2.Shannxi Key Laboratory of Phytochemistry,Baoji 721013,China;3.Baoji Hospital of Traditional Chinese Medicine,Baoji 721004,China)  
Adopting single factor trial,the orthogonal test was designed to optimizate the extraction conditions of total amino acids from Lobaria kurokavae Yoshim.According to the output of total amino acids under the different extraction conditions,which were selected as solvent concentration,ratio of liquid to material,extraction temperature and extraction time for extraction process.Results showed that the ratio of liquid to material was the main factor on the extraction process.The optimum conditions were established as follows: the concentration of alcohol was 60%,volume of alcohol was 20 times amount of herb,and the herb was extracted 3hours with temperature 70℃.
【Fund】: 陕西省教育厅重点实验室基金资助项目(08JZ08);; 宝鸡文理学院院级重点项目(ZK0844)
【CateGory Index】: TQ461
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