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《Acta Chinese Medicine and Pharmacology》 2011-01
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Determination of Uronic Acid Contents in Polysaccarides from the Stems of Ephedra Sinica Stapf

XIA Yong-gang,LIANG Jun,YANG Bing-you,WANG Qiu-hong,KUANG Hai-xue(Key Laboratory of Chinese Materia Medica(Heilongjiang University of Chinese Medicine),Ministry of Education,Heilongjiang key Laboratory of TCM Pharmacodynamic Material Bases,Harbin 150040,China)  
Objective: To determine the uronic acid contents of the polysaccharides from the stems of Ephedra sinica stapf.Methods: The contents of uronic acid in polysaccharide were measured by carbazole and sulphuric acid method.Results: The content of uronic acid in Mahuang polysaccharide was 45.89%,and its recovery rate was 101.10%.RSD was 1.42%.Conclusion: This method is simple and accurate.It will provide an excellent quantitative method and reference for quality control of ephedra sinica stapf.
【Fund】: 十二五国家科技部重大专项课题“重大新药创制”专项课题(编号:2011ZX09102-006-01);; 国家自然科学基金面上项目(编号:30973870);; 黑龙江中医药大学博士创新基金(编号:B201002)
【CateGory Index】: R284.1
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