Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Traditional Chinese Drug Research & Clinical Pharmacology》 2007-03
Add to Favorite Get Latest Update

In-vitro Inhibitory Effect of Phyllanthus Urinaria L Compound on Proliferation of Human liver Cancer Cell HePG_2 and Its Apoptosis Induction

LU Yuhui,FU Linchun(Institute of Tropic Medicine,Guangzhou University of TCM,Guangzhou 510405)  
Objective To investgate the inhibitory effect of Phyllanthus Urinaria L compound on proliferation of hepatoma cell HePG2 in vitro and to explore its mechanism.Methods The influence of different concentrations of Phyllanthus Urinaria L compound at different time on HePG2 proliferation was compared by MTT colorimetric assay and cell growth curve assay.The cell apoptotic rate and morphological changes of HePG2 were observed by flow cytometry,fluorescence microscope and electron microscope.Results Phyllanthus Urinaria L compound can inhibit the proliferation of human hepatoma cell line HePG2.Within a certain limit of concentrations,the higher the concentration and the longer the acting time,the stronger the inhibition.Co-cultured with 500 μ g/mL Phyllanthus Urinaria L compound for 72 h,the inhibitory rate reached 93.58 % and IC50 was 240 μ g/mL.Phyllanthus Urinaria L compound at different concentrations had an certain effect on inducing cell apoptosis.Conclusion Phyllanthus Urinaria L compound can inhibit the proliferation of hepatoma cell,and its mechanism may be related with the induction of hepatoma cell HePG2 apoptosis.
【Fund】: 广州市科技计划项目资助(K4030003)
【CateGory Index】: R285.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 Lu Yuhui Chen Wenyuan Fujian University of TCM,Fuzhou 350108;Effects of Phyllanthus urinaria L. Compound Recipe on Proliferation and Apoptosis and Signal Transduction Pathway of HePG_2[J];Guangming Journal of Chinese Medicine;2010-03
2 SUN Zhen1,WANG Zhong1,YU Zheng-hong2 reviewing,LING Chang-quan3 checking(1.Department of Sanitarium Area,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing 211131,Jiangsu,China;2.Department of Oncology,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing 210002,Jiangsu,China;3.Department of Traditional Chinese Medicine,Changhai Hospital,Second Military Medical University,Shanghai 200433,China);Research in progress of compound Chinese medicine preparations on preventing the hepatocellular carcinoma[J];Journal of Medical Postgraduates;2011-05
【Citations】
Chinese Journal Full-text Database 2 Hits
1 YANG Jin nan1* , PAN Qi chao1, ZHOU Ying2, LIANG Yong ju1, FU Li wu1 1.Cancer Research Institute,Sun Yat sen University of Medical Sciences,Guangzhou 510060, P.R.China 2.Chinese Medicine Research Insitution of Guangxi,Nannin 530022,P.R.China;Antitumor effect of diacetyldianhydrogalactitol and its mechanism[J];Chinese Journal of Cancer;2000-12
2 LU Jian - hua, CHEN Jian - guo, NI Zheng - ping, et al. Qidong Liver Cancer Institute, Jiangsu 226200, China;A Twenty- two Year Prospective Study on HBsAg Carrier State and Primary Liver Cancer in a Community, Jiangsu[J];China Preventive Medicine;2002-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WEI Yi-yi, LAI Guo-li, DENG Zhen-de, et al.Guangxi Medical University (Nanning 530021) China;Study on the growth effect of 1,2:5,6-Dianhydro-3,4-diacetylgalactitol on human hepatocellular carcinoma[J];Guangxi Medical Journal;2005-02
2 CHEN Jian-guo.Department of Epidemiology,Qidong Liver Cancer Institute,Qidong 226200,China;Advances in the Etiology and Prevention for Primary Liver Cancer[J];Journal of Qilu Oncology;2003-11
3 SHEN Jian-ke,LI Chang-qing.LIANG Man-yi,et al.(Institute of Tropical Medicine,Guangzhou Traditional Chinese Medicine University,Guangzhou 510405,Guangdong,P.R.China);Inhibitory effect of compound phyllanthus urinaria Ⅱ (CPU Ⅱ) on proliferation of human hepatocellur carcinoma HepG_2 and induced apoptosis in vitro[J];China Tropical Medicine;2011-07
4 Dong-Sheng Yuan, Professional Technology College, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China Xin-Hua Wang, International College, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China Chang-Qing Li, Tropical Medicine Institute, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China Hui-Quan Xiao, First Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China;Anti-HBV effect of compound Ganaining in vitro[J];World Chinese Journal of Digestology;2004-06
5 Dong-Sheng Yuan, Professional Technology College, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China Xin-Hua Wang, Headmaster's Office, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China Chang-Qing Li, Ni Liu, Tropical Medicine Institute, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China;Apoptosis of human hepatocellular carcinoma cell line HepG_2 induced by composite Gan'aining medicated serum in vitro[J];World Chinese Journal of Digestology;2006-05
6 Dong-Sheng Yuan Dong-Hui Liu Professional Technology College,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,Guangdong Province,China Xin-Hua Wang Headmaster's Office,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,Guangdong Province,China Cai-Ping Zhu Lei Shi Postgraduate of Grade 2005,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,Guangdong Province,China;Inhibitory effect of composite Gan'aining on growth and alpha-fetoprotein secretion of human hepatoma cells[J];World Chinese Journal of Digestology;2006-19
7 DU Yan-ping ZHU Hui-lian(Epartment of Nutrition,School of public health,Sun Yat-sen university 74~(th) Zhongshan Road II,Guangzhou,510080);The possible mechanism of Aflatoxin B_1 Hepatocarcinogenesis and preventive effects of phytochemicals[J];Journal of Modern Food and Pharmaceuticals;2006-01
8 ZHANG Qinan,LU Peixin,WANG Jinbing,et al.Liver Cancer Resuorch Institute of Qidong,Nantong 226200, CHINA;Analysis of twenty-year survey on the population at high risk of developing primary hepatic carcinoma[J];Jiangsu Medical Journal;2008-12
9 PENG Li-sheng,HE Jing-song,TONG Guang-dong,et al.Department of Hepatology,Shenzhen Hospital of Traditional Chinese Medical(Shenzhen Guangdong,518003)China;Inhibition of extract of phyllanthus urinaria L.on hepatitis B virus and hepatitis B X gene in vitro[J];Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases;2006-06
10 JIANG Fei,CHEN Zeng-rui.(Health Bureau of Yuhuan,Yuhuan,Zhejiang,317600,China.);A Case-control Study on Influencing Factors of Liver Cancer with 112 Cases[J];Zhejiang Journal of Preventive Medicine;2008-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Xiao-zhong, CHEN Xiao-chun, YANG Ying-hong, CHEN Zhi-xin HUANG Yue-hong, TAO Qi-min (Department of Gastroenterology, Union Hospital, Fujian Medical University, Fuzhou 350001, P. R. China; Department of pathology, Union Hospital, Fujian Medical U;Expression of HBxAg and Fas/FasL in Patients with Hepatocellular Carcinoma[J];Chinese Journal of Cancer;2001-01
2 Cell Line K562/A02 WANG Jue-Qiong1, CHEN Bao-An1, CHENG Jian1, XU Wen-Lin2, SUN Xin-Chen3 1. Department of Hematology, Zhongda Hospital, Southeast University, Nanjing,Jiangsu, 210009, P. R. China 2. Department of Hematology, People's Hospital, Jiangsu University, Zhenjiang, Jiangsu, 212002, P. R. China 3. Department of Oncology, Zhongda Hospital, Southeast University, Nanjing, Jiangsu, 210009, P. R. China;Comparison of Reversal Effects of 5-Bromotetrandrine and Tetrandrine on P-Glycoprotein-dependent Resistance to Adriamycin in Human Lukemia[J];Chinese Journal of Cancer;2008-05
3 QIAN Xiao-Ping,LIU Bao-Rui,HU Jing,LI Min,HU Wen-Jing,SUN Jing,YU Li-Xia Cancer Center,Drum Tower Hospital,Medical School of Nanjing University,Nanjing,Jiangsu,210008,P.R.China;Inhibitory Effect of Tetrandrine on Angiogenesis[J];Chinese Journal of Cancer;2008-10
4 LIU Xiao-li,ZAN Deng,YUAN Jing-jue,CAI Dong-lian,ZHENG Hui(Clinical Nutrition Center,Changhai Hospital,the Second Military Medical University,Shanghai 200433,China);Effects of Zymosan on Antioxidation and Immune Function In S_(180) Tumor-Bearing Mice[J];Amino Acids & Biotic Resources;2009-04
5 Ye Yong1,Zhang Yongbo1,Zhu Quanhong2 1.Chemical Engineering College,South China University of Technology,Guangzhou(510540) 2.TCM College,Southern Medical University;Separation and Determination of Polyphenols in Phyllantuus Urinaria L.by LC/MS[J];Journal of Zhejiang University of Traditional Chinese Medicine;2008-04
6 LIU Kai-ge,GAO Hong-yan,ZHAO Hui,NIU Chun-yan,LI Li(Department of Digestive Diseases,Affiliated Hospital,Xi'an Medical College,Xi'an 710077,China);Correlation between HBx and VEGF expressions and angiogenesis and metastasis in HBV related hepatocellular carcinoma tissue[J];Journal of Jilin University(Medicine Edition);2010-02
7 HU Qun 1,NING Tao 1,SU Xiu Lan 2,WANG Wen Li 2,KE Yang 1 (1. Laboratory of Cytogenetics, Beijing Institute for Cancer Research, Peking University School of Oncology, Beijing 100034, China; 2. Inner Mongolia Medical College);Improvement of chick chorioallantoic membrane technique[J];Journal of Beijing Medical University;2001-04
8 HU Bing, AN Hong mei, LI Xin min (胡兵,等) The First people Hospital of Chenzhou (Chenzhou,423000,China);The Study of Chinese Medicine Compound/DPC Induced Differentiation on Human Heptocarcinoma Cell Lines HCC 9204 and It's Mechanism[J];JOURNAL OF CHENGDU UNVERSITY OF TARDITIONAL CHINESE MEDICINE;2000-01
9 LIU Chuan,WANG Chun,LIU Ping,et al (The internal medicine of neurology,The people's hospital of DeYang city,Sichuan DeYang,618000);Analyze on Curative Effect of PTS in Treatment of 116 Cases of Cerebral Infaction[J];Journal of Chengdu University of Traditional Chinese Medicine;2007-01
10 WU Sheng-xi1,2,LU Chun-li1,ZENG Jin-lan1,CHEN Yong-wen2,YANG Cheng-yin2,GUO Sheng2,FEI Lei2,WU Yu-zhang2(1.School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400050,China;2.Department of immunology,Third Military Medical University,Chongqing 400017,China);The Research Progress in Hepatitis B Virus X Protein[J];Journal of Chongqing University of Technology(Natural Science);2010-11
【Secondary References】
Chinese Journal Full-text Database 5 Hits
1 TANG Hao,TANG An-fu,CUI En-zhong reviewing,WANG Shu-dong checking(Department of Pharmaceutical Preparation,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing 210002,Jiangsu,China);Influencing factors on the ZTC 1+1 natural clarifier in the preparation of traditional Chinese medicine[J];Journal of Medical Postgraduates;2012-03
2 WANG Xin-xing1,ZHANG Luo1,SUN Zhen2 reviewing,YU Zheng-hong1 checking(1.Department of Oncology,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing 210002,Jiangsu,China;2.Tangshan Sanitarium,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing 211131,Jiangsu,China);Evil-expelling Chinese drugs for primary hepatic carcinoma[J];Journal of Medical Postgraduates;2012-12
3 JIANG Jingrong,ZHANG Tianhong,ZHONG Sen△(Department of Infectious Diseases,Affiliated Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610000,China);Clinical Application and the Mechanism of Phyllanthus for Chronic Hepatitis B[J];Journal of Practical Traditional Chinese Internal Medicine;2013-04
4 ZHANG Yi ZHOU Rong-yao ZHONG Yi ZHANG Yan-bo LAI You-ying PAN Xin Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine;Effect of "Bushen Jianpi Decoction" Drug Serum on Proliferation and Apoptosis of Human Hepatoma Cell Line SMMC-7721[J];Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai;2013-03
5 Chen Peifeng,Pan Lei,Jin Yingqi.Department of Oncology,The First Affiliated Hospital of Zhejiang Chinese Medical University,Zhejiang 310006,China;Proliferation and Apoptosis Effect on Human Umbilical Vein Endothelial Cell Induced by Human Lung Adenocarcinoma Cells Treated with Qingrexiaoji Recipe[J];Journal of Medical Research;2013-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved