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《Traditional Chinese Drug Research & Clinical Pharmacology》 2008-02
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Study on Antioxidant Stilbenes from the Rhizomes of Smilax china

ZHAO Zhongxiang1, JIN Jing3, ZHU Chenchen1, ZHANG Cuixian1, RUAN Jinlan2 (1. School of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006; 2. College of Pharmacy, Tongji Medical Center, Huazhong University of Science and Technology, Wuhan 430030; 3. School of Pharmaceutical Sciences, Zhongshan University, Guangzhou 510080)  
Objective To study the stilbene constituents from the traditional Chinese medicine Smilax china and to determine their antioxidant activity. Methods The compounds were separated and purified by column chromatography with silica gel, RP C18, and Sephadex LH- 20, and were identified by IR, MS, NMR. DPPH method was used to evaluate the free radical scavenging activity of the isolated compounds. Results Three compounds were isolated from the EtOAc fraction of the rhizomes of S. china and were identified as: resveratrol (1), oxyresveratrol (2) and 3, 5, 3′ , 4′ - tetrahydroxylstilbene (3). Compounds 1~ 3 showed strong antioxidant activity, and could scavenge DPPH free radicals, effectively. At the concentration of 50 μ mol/L, their DPPH free radical scavenging rates were 79.47 % , 89.89 % and 93.86 % , respectively. Conclusion Stilbenes might be the material foundation of antioxidant activities of rhizomes of S. china.
【CateGory Index】: R284
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