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Determination of Total saponins and Ziyuglycoside Ⅰ in Sanguisorba officinalis L.

ZHANG Xuewen1,WEI Wei1,CHENG Yue1,LIU Haowen1,CHEN Jianping2,WANG Dongmei1(1.School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006 Guangdong,China;2.School of Chinese Medicine,the University of Hong Kong,Hong Kong,China)  
Objective To establish the quantification method for total saponins and ziyuglycoside I,and to determine their contents in different batches of Sanguisorba officinalis L.Methods The ultraviolet-visible spectrophotometer was optimized for the determination of total saponins,and HPLC-ELSD was employed for the determination of ziyuglycoside I in S.officinalis,respectively.Results The coloration system for the determination of total saponins was vanillin-acetic acid-perchloric acid.The condition of coloration was 15 % vanillin,1 mL perchloric acid,80 ℃ water bathed for 15 min,and the detection wavelength was 549 nm.In seven batches of commercial crude drugs of S.officinalis,the contents of total saponins were measured as 7.25%~8.41 %,and the contents of Ziyu-glycoside I were 5.21 %~ 6.18 %.Conclusion The developed methods are simple,rapid,accurate,stable,and reproducible with high selectivity,and are suitable for the determination of total saponins and ziyuglycoside I in the quality control of S.officinalis,respectively.
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