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《Chinese Journal of Birth Health & Heredity》 2008-04
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An experiment of extracting phytohemagglutinin(PHA) from small white bean

HE Zai-qing,LIU Yan-ming.(Department of Pathology,Nuclear Industry 416 Hospital,Chengdu 610051,Sichuan)  
Objective: Try to extract PHA form small white beans which is sold in market.Methods:Fine powder of small white bean were marinated in 0.9% normal saline for 48 hours.The centrifugalized and filtered extract is tested in the trial of lymphocyte chromosome cultivation,compared with the PHA from Yi Hua company,checking its evaluation as mitogen.Results:The PHA extracted from white small bean,similar to the PHA from Yi Hua company,can stimulate lymphocyte transforming to lymphoblast.Conclusion: The PHA extracted from white small bean can settle for preparation of chromosome in normal laboratory condition.
【CateGory Index】: Q946
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