Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Pharmacology and Clinics of Chinese Materia Medica》 2005-04
Add to Favorite Get Latest Update

Establishment and application of a non-isotope model in vitro for screening 5α-reductase inhibitor

Kuang Gang,Zou Wenjun,Gao Xiaoping1(Chengdu University of TCM, Chendu 610075;1 New Drug Screening Center of Chengdu Diao Group, Chendu 610041)  
For establishing a non-isotope model in vitro for screening 5alpha-reductase inhibitor ,the 5alpha-reductase abstracted from the prostates of male rats and testosterone as a substrate together with nieotinamide adenine dinueleotide phosphare (NADPH) as a hydrogen supplier, were composed of a minim reactive system. The production dihydrotestosterone (DHT) was measured by the enzyme immunoassay(EIA). The activity of enzyme was determined by the quantity of production. We optimized the concentrations of substrate, NADPH and enzyme, temperature as well as time. When the concentrations of substrate,NADPH ,enzyme were 0.25μM,1mM and 0.3mg respectively, the maximum activity of enzyme was achieved at 37℃ after 60min reaction. We established a positive control of specific 5alpha-reductase inhibitor by finasteride. The result showed it obviously reduced the production of DHT, which indicated that the activity of 5alpha-reductase was inhibited. Further more we screened 24 herbal abstracts and found some had the function similar to 5 alpha-reductase inhibitor, such as Jiang Huang (姜黄) and Xiakucao (夏枯草). The above result proved that the non-isotope method in this study is more effective and practical, and can be used as a non-isotope model in vitro for screening 5alpha-reductase inhibitor.
【CateGory Index】: R96
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 4 Hits
1 YU Kai(Bioengineer College of Southwest Jiaotong University,Chengdu 610031,China);Inhibition of Pterocarpus indicus Willd extract on 5α-reductase[J];West China Journal of Pharmaceutical Sciences;2007-05
2 WU Wen-zhe,CHENG Zhi-hong,YU Chui-liang,HOU Hui-min Shanghai Institute of Pharmaceutical Industry & National Pharmaceutical Engineering Research Center, Shanghai 201203,China);Screening 5a-reductase Inhibitors from Plant Extraction for Treatment of Androgenic Alopecia in vitro[J];Research and Practice on Chinese Medicines;2010-02
3 YAN Dong-mei TU Ling-lan LI Wen-jun (Zhejiang Academy of Medical Sciences,Hangzhou 310013,China);A model to screen 5α-reductase inhibitors in vitro by RP-HPLC[J];Chinese Journal of Pharmaceutical Analysis;2008-07
4 LIU Ben~1,CHANG Yi-ling~2,CHEN Ke-li~3(1.Ningbo School of Technology,Zhejiang University,Ningbo 315100,China;2.Zhejiang Pharmaceutical College,Ningbo 315100,Chian;3.Hubei College of Traditional Chinese Medicine,Wuhan 430061,China);Progress in the Study of 5α-Reductases Used as A Target in Natural Medicine Screening[J];Pharmaceutical Biotechnology;2006-06
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Hong Jun WANG 1 , Jia Ying ZHANG 2 , Su Huan LIU 1 , Hui ZHAO 1 , Zhong Shan WANG 1 (1 Department of Cell Biology, 2 the Second General Laboratory, School of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, China);Changes of 5 Alpha-reductase Type Ⅱ Activity in Sexual Gland of Diabetic Male Rats[J];Acto andrologica Sinica;2003-02
2 JIA Yue, WANG Xiao dong, CUI Yu gui, DI Fu song;Roles of 5 α-reductase in male reproduction[J];Journal of Reproductive Medicine;2003-02
【Co-citations】
Chinese Journal Full-text Database 9 Hits
1 GU Yun-lan~1, LI Bao-zong~2, GU Jia~2(1. Chemistry Department, YanCheng Teachers' College, YanCheng, 224002, China;2. College of Chemistry and Chemical Engineering, Suzhou University, Suzhou, 215006);Study on the Relationships Between the Structure and Inhibition Activity of 17β-N-X-4-Azasteroids Toward SRD5A2[J];Journal of Anqing Teachers College(Natural Science Edition);2004-04
2 LI Xiao mei,LU Yan juan,LI Xiao lin * (Experimental Center of Biochemistry and Molecular Biology,School of Basic Medical Sciences, Jilin University, Changchun 130021,China);Changes of POD activities in the key organs in adult rats with diabetes[J];Journal of Jilin University of (Medicine Edition);2004-01
3 LU Yan juan, LI Xiao lin , LI Xiao mei (Experimental Center of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Jilin University, Changchun 130021,China);Changes of levels of GSH-Px, GSH,and GR in important organs and tissues of adult diabetic rats[J];Journal of Jilin University of (Medicine Edition);2004-03
4 JIA Yue 1, CUI Yu-gui 2, WANG Xiao-dong 1, WANG Xing-hai 3, TONG Jian-sun 3, MA Ding-zhi 3, CAI Rui-fen 3, DI Fu-song 1 1.Department of Endocriology; 2.Key Laboratory, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China; 3.Jiangsu Family Planning Institute, Nanjing, Jiangsu 210029, China;Effect of Selective 5α-Reductase Inhibitor or/and Testosterone Undecanoate on the Reproductive Function of Male Rats[J];Nationlal Journal of Andrology;2005-01
5 WANG Xiao-dong~(1),JIA Yue~(1),CUI Yu-gui~(1),WANG Xinghai~(2),TONG Jian-sun~(2),MA Ding-zhi~(2),CAI Rui-fen~(2),DI Fu-song~(1)1.Department of Endocrinology,the First Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China;2.Jiangsu Institute for Family Planning,Nanjing,Jiangsu 210029,China;Effect of Large-dosage of 5α-reductase Inhibitors on the Spermatogenesis of Male Rats[J];Nationlal Journal of Andrology;2005-09
6 JI Bao-quan,YANG Ai-gang,ZHU Yong-ning,PANG Yue,TANG Ling,FENG Bao-min,WANG Yong-qi(College of Bio-engineering,Dalian University,Dalian 116622,China);Study the 5α-reductase Inhibitory Activity of Urtica fissa E. Pritz's Extract[J];Lishizhen Medicine and Materia Medica Research;2009-08
7 YAN Dong-mei TU Ling-lan LI Wen-jun (Zhejiang Academy of Medical Sciences,Hangzhou 310013,China);A model to screen 5α-reductase inhibitors in vitro by RP-HPLC[J];Chinese Journal of Pharmaceutical Analysis;2008-07
8 LI Xiao-Lin, LI Xiao-Mei, LU Yan-Juan, et al . Experimental Center for Biochemistry and Molecular Biology, Basic Medical College of Jilin University, Changchun 130021, Jilin, China;The content changes of SOD and MDA in important organic tissues of adult diabetic rat[J];Chinese Journal of Gerontology;2005-04
9 DING Wei, ZOU Da-jin(Changhai Hospital affiliated to The Second Military Medical University,department of endocrinology, Shanghai 200433, China);The Progress of the Research on the Key Role of 5a-reductase in Androgen-induced Acne[J];The Chinese Journal of Dermatovenereology;2009-08
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Yong-qi,FENG Bao-min,LI Fan,LIU Jing-yan,YANG Ai-gang,PANG Yue,MU Hong-mei(Drug Researching Institute,Dalian University,Dalian 116622,China);Review on the research of BPH treatment by urtica plants[J];Journal of Dalian University;2005-02
2 YANG Shu-hui1,TIAN Xuan1,2*(1 State Key Laboratory of Applied Organic Chemistry,Lanzhou University,Lanzhou 730000,China;2 Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin Xinjiang Production & Construction Corps,Tarim,Xinjiang 843300,China);Chemical Components of Rhododendron concinnum[J];Acta Botanica Boreali-Occidentalia Sinica;2007-02
3 OU Min-rui~1, DAI Xiao-fu~1, XUE Feng-chun~1, ZHANG Yi~2, XU Xiao-ping~1 (1. College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, Fujian 350002, China; 2. Office of Educational Administration, Fuzhou University, Fuzhou, Fujian 350002, China);Analysis on the activity of 5α-reductase using high performance liquid chromatography[J];Journal of Fuzhou University(Natural Sciences Edtion);2004-02
4 CHEN Jie-bo,XU Xiao-ping,OU Min-rui,XIE Jun (College of Chemistry and Chemical Engineering,Fuzhou University,Fuzhou,Fujian 350002,China);Screen steroid 5 alpha-reductase inhibitors in vitro by high performance liquid chromatography[J];Journal of Fuzhou University(Natural Sciences Edtion);2005-05
5 RUI Caixia,YANG Xiaoming Beijing Institute of Radiation Medicine, Beijing, 100850, China;Association of Steroid 5α-reductase with Hormone-associated Tumour[J];Journal of Medical Molecular Biology;2006-04
6 OU Min rui 1,DAI Xiao fu 1,ZHOU Xun sheng 2,CHEN Zan qiu,XU Xiao ping(1.College of Chemistry and Chemical Engineering; 2. Office of Educational administration, Fuzhou University, Fuzhou, Fujian 350002, China);Summarization on the Study of 5α-reductase and its Inhibitors[J];Strait Pharmaceutical Journal;2003-06
7 YU Kai(Bioengineer College of Southwest Jiaotong University,Chengdu 610031,China);Inhibition of Pterocarpus indicus Willd extract on 5α-reductase[J];West China Journal of Pharmaceutical Sciences;2007-05
8 ZHOU Li-qun;Etiopathogenisis、Diagnosis and Therapy Progress of Prostatic Carcinoma[J];Continuing Medical Education;2006-08
9 Qu Fengyu et al;Effects of different extracts of rehannia glutinosa libosch on the brain SOD and MDA of D-galactose-induced senile mice[J];Heilongjiang Medicine and Pharmacy;1998-05
10 Hong Jun WANG 1 , Jia Ying ZHANG 2 , Su Huan LIU 1 , Hui ZHAO 1 , Zhong Shan WANG 1 (1 Department of Cell Biology, 2 the Second General Laboratory, School of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, China);Changes of 5 Alpha-reductase Type Ⅱ Activity in Sexual Gland of Diabetic Male Rats[J];Acto andrologica Sinica;2003-02
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 DANG Juan,YU Kai*,WANG Yao(Bioengineering College,Southwestern Jiaotong University,Chengdu,Sichuan,610031 P.R.China);Study on treatment of Pterocarpus indcus Willd extracts on rat androgenetic alopecia[J];West China Journal of Pharmaceutical Sciences;2010-03
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 LIU Su-huan,LI Zhe,ZUO Wen-jing,et al(Deptarment of Cell Biology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China);Changes of the Weight and Coefficient of Sexual Gland and the Level of Sexual Hormone in Adult Diabetic rat[J];Jilin Medical Journal;2002-05
2 Yu-Gui CUI1, Gui-Yuan ZHANG2 (1. Department of Endocrinology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China; 2. National Research Institute of Family Planning, Beijing 100081, China);Steroid 5α-Reductase Activity, Androgen Level and Its Receptor in the Target Tissues of Male Fetuses[J];Acto andrologica Sinica;2001-04
3 Wang Zhong Shan, et al Norman Bethune University of Medical Sciences(Changchun 130021);Observation on sexual organ weight and behavior in pubertal rats with diabetes[J];;1996-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved