Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Pharmacology and Clinics of Chinese Materia Medica》 2005-04
Add to Favorite Get Latest Update

Establishment and application of a non-isotope model in vitro for screening 5α-reductase inhibitor

Kuang Gang,Zou Wenjun,Gao Xiaoping1(Chengdu University of TCM, Chendu 610075;1 New Drug Screening Center of Chengdu Diao Group, Chendu 610041)  
For establishing a non-isotope model in vitro for screening 5alpha-reductase inhibitor ,the 5alpha-reductase abstracted from the prostates of male rats and testosterone as a substrate together with nieotinamide adenine dinueleotide phosphare (NADPH) as a hydrogen supplier, were composed of a minim reactive system. The production dihydrotestosterone (DHT) was measured by the enzyme immunoassay(EIA). The activity of enzyme was determined by the quantity of production. We optimized the concentrations of substrate, NADPH and enzyme, temperature as well as time. When the concentrations of substrate,NADPH ,enzyme were 0.25μM,1mM and 0.3mg respectively, the maximum activity of enzyme was achieved at 37℃ after 60min reaction. We established a positive control of specific 5alpha-reductase inhibitor by finasteride. The result showed it obviously reduced the production of DHT, which indicated that the activity of 5alpha-reductase was inhibited. Further more we screened 24 herbal abstracts and found some had the function similar to 5 alpha-reductase inhibitor, such as Jiang Huang (姜黄) and Xiakucao (夏枯草). The above result proved that the non-isotope method in this study is more effective and practical, and can be used as a non-isotope model in vitro for screening 5alpha-reductase inhibitor.
【Key Words】: 5alpha-reductase activity drug screening finasteride
【CateGory Index】: R96
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 8 Hits
1 YU Kai(Bioengineer College of Southwest Jiaotong University,Chengdu 610031,China);Inhibition of Pterocarpus indicus Willd extract on 5α-reductase[J];West China Journal of Pharmaceutical Sciences;2007-05
2 WU Wen-zhe,CHENG Zhi-hong,YU Chui-liang,HOU Hui-min Shanghai Institute of Pharmaceutical Industry & National Pharmaceutical Engineering Research Center, Shanghai 201203,China);Screening 5a-reductase Inhibitors from Plant Extraction for Treatment of Androgenic Alopecia in vitro[J];Research and Practice on Chinese Medicines;2010-02
3 WU Chuanling,MA Songtao(School of Pharmacy,Chengdu Medical College,Chengdu 610083,Sichuan,China);5α-reductase Inhibitors Screening Model in Vitro[J];Journal of Liaoning University of Traditional Chinese Medicine;2013-07
4 YAN Dong-mei TU Ling-lan LI Wen-jun (Zhejiang Academy of Medical Sciences,Hangzhou 310013,China);A model to screen 5α-reductase inhibitors in vitro by RP-HPLC[J];Chinese Journal of Pharmaceutical Analysis;2008-07
5 LIU Ben~1,CHANG Yi-ling~2,CHEN Ke-li~3(1.Ningbo School of Technology,Zhejiang University,Ningbo 315100,China;2.Zhejiang Pharmaceutical College,Ningbo 315100,Chian;3.Hubei College of Traditional Chinese Medicine,Wuhan 430061,China);Progress in the Study of 5α-Reductases Used as A Target in Natural Medicine Screening[J];Pharmaceutical Biotechnology;2006-06
6 Wu Chuanling,Ma Songtao,Wang Fuqin,Lan Xiaoping,Meng Lijuan(School of Pharmacy,Chengdu Medical College,Chengdu,Sichuan,China 610083);Study of Inhibiting Effect of Lotus Extract on 5α-Reductase[J];China Pharmaceuticals;2012-10
7 Zhou Benhong~(1,2),Guo Min~1,Wu Lining~2,Chen Dong~2(1.Department of Pharmacy,Renmin Hospital of Wuhan University,Wuhan 430060,China;2.School of Pharmaceutics Science,Wuhan University);UV-spectrophotometric Screening of 5α-Reductase Inhibitors in vitro[J];China Pharmacist;2011-12
8 WANG Yang(Maanshan Municipal Hospital Group,Anhui Maanshan 243000,China) WANG Xing-sheng(School of Pharmacy,Anhui University of Traditional Chinese Medicine,Hefei 230038,China);Establishment and Application of in Vitro Novel Selection Model for hSRD5A2 in Drug Selection[J];China Pharmacy;2012-31
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Hong Jun WANG 1 , Jia Ying ZHANG 2 , Su Huan LIU 1 , Hui ZHAO 1 , Zhong Shan WANG 1 (1 Department of Cell Biology, 2 the Second General Laboratory, School of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, China);Changes of 5 Alpha-reductase Type Ⅱ Activity in Sexual Gland of Diabetic Male Rats[J];Acto andrologica Sinica;2003-02
2 JIA Yue, WANG Xiao dong, CUI Yu gui, DI Fu song;Roles of 5 α-reductase in male reproduction[J];Journal of Reproductive Medicine;2003-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 GU Yun-lan~1, LI Bao-zong~2, GU Jia~2(1. Chemistry Department, YanCheng Teachers' College, YanCheng, 224002, China;2. College of Chemistry and Chemical Engineering, Suzhou University, Suzhou, 215006);Study on the Relationships Between the Structure and Inhibition Activity of 17β-N-X-4-Azasteroids Toward SRD5A2[J];Journal of Anqing Teachers College(Natural Science Edition);2004-04
2 LI Xiao mei,LU Yan juan,LI Xiao lin * (Experimental Center of Biochemistry and Molecular Biology,School of Basic Medical Sciences, Jilin University, Changchun 130021,China);Changes of POD activities in the key organs in adult rats with diabetes[J];Journal of Jilin University of (Medicine Edition);2004-01
3 LU Yan juan, LI Xiao lin , LI Xiao mei (Experimental Center of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Jilin University, Changchun 130021,China);Changes of levels of GSH-Px, GSH,and GR in important organs and tissues of adult diabetic rats[J];Journal of Jilin University of (Medicine Edition);2004-03
4 JIA Yue 1, CUI Yu-gui 2, WANG Xiao-dong 1, WANG Xing-hai 3, TONG Jian-sun 3, MA Ding-zhi 3, CAI Rui-fen 3, DI Fu-song 1 1.Department of Endocriology; 2.Key Laboratory, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China; 3.Jiangsu Family Planning Institute, Nanjing, Jiangsu 210029, China;Effect of Selective 5α-Reductase Inhibitor or/and Testosterone Undecanoate on the Reproductive Function of Male Rats[J];Nationlal Journal of Andrology;2005-01
5 WANG Xiao-dong~(1),JIA Yue~(1),CUI Yu-gui~(1),WANG Xinghai~(2),TONG Jian-sun~(2),MA Ding-zhi~(2),CAI Rui-fen~(2),DI Fu-song~(1)1.Department of Endocrinology,the First Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China;2.Jiangsu Institute for Family Planning,Nanjing,Jiangsu 210029,China;Effect of Large-dosage of 5α-reductase Inhibitors on the Spermatogenesis of Male Rats[J];Nationlal Journal of Andrology;2005-09
6 JI Bao-quan,YANG Ai-gang,ZHU Yong-ning,PANG Yue,TANG Ling,FENG Bao-min,WANG Yong-qi(College of Bio-engineering,Dalian University,Dalian 116622,China);Study the 5α-reductase Inhibitory Activity of Urtica fissa E. Pritz's Extract[J];Lishizhen Medicine and Materia Medica Research;2009-08
7 YAN Dong-mei TU Ling-lan LI Wen-jun (Zhejiang Academy of Medical Sciences,Hangzhou 310013,China);A model to screen 5α-reductase inhibitors in vitro by RP-HPLC[J];Chinese Journal of Pharmaceutical Analysis;2008-07
8 DING Wei, ZOU Da-jin(Changhai Hospital affiliated to The Second Military Medical University,department of endocrinology, Shanghai 200433, China);The Progress of the Research on the Key Role of 5a-reductase in Androgen-induced Acne[J];The Chinese Journal of Dermatovenereology;2009-08
9 HE Lian,CHEN Hai-Tao,YANG Xin-Hai,et al.Department of Surgeon,Maternal and Child Health Care Hospital of Hubei,Wuhan 430071,Hubei,China;The effect of hCG on micropenis of prepubertal children with hypogonadotropic hypogonadism[J];Maternal and Child Health Care of China;2011-20
10 LI Xiao-Lin, LI Xiao-Mei, LU Yan-Juan, et al . Experimental Center for Biochemistry and Molecular Biology, Basic Medical College of Jilin University, Changchun 130021, Jilin, China;The content changes of SOD and MDA in important organic tissues of adult diabetic rat[J];Chinese Journal of Gerontology;2005-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Yong et al(School of Life Sciences and Technology,North University for Ethnics,Yinchuan,Ningxia 750021);Comparison and Evaluation of Methods for Licorice Flavonoids Content Determination[J];Journal of Anhui Agricultural Sciences;2009-28
2 HE Zhi-ping et al(School of Agriculture and Food Science,Zhejiang Forestry University,Hangzhou,Zhejiang 311300);Research Progress on the Detection Methods of Lipid Oxidation[J];Journal of Anhui Agricultural Sciences;2010-21
3 LIU Li-xiang1, LIANG Xing-fei1, SUN Yi1, Tanguy Laura1,2, YE Hong1, ZENG Xiao-xiong1* (1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. Department of Food Engineering, Polytech’Lille, 59655 Villenevve d’Asqc, France);Determination of Polyphenolic Content and Antioxidant Activity of Kudingcha Made from Ilex kudincha C. J. Tseng[J];Journal of Tea Science;2008-04
4 WANG Yong-qi,FENG Bao-min,LI Fan,LIU Jing-yan,YANG Ai-gang,PANG Yue,MU Hong-mei(Drug Researching Institute,Dalian University,Dalian 116622,China);Review on the research of BPH treatment by urtica plants[J];Journal of Dalian University;2005-02
5 YANG Shu-hui1,TIAN Xuan1,2*(1 State Key Laboratory of Applied Organic Chemistry,Lanzhou University,Lanzhou 730000,China;2 Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin Xinjiang Production & Construction Corps,Tarim,Xinjiang 843300,China);Chemical Components of Rhododendron concinnum[J];Acta Botanica Boreali-Occidentalia Sinica;2007-02
6 ZHOU Jing,WANG Bo-chu,WANG Yang(College of Bioengineering,Chongqing University,Key Laboratory for Biomechanics & Tissue Engineering Under the State Ministry of Education,Chongqing 400030,China);Determination and Evaluation of Saccharides Content in Extract of Herba Spirodelae Seu Lemnae Polysaccharides[J];Journal of Chongqing University(Natural Science Edition);2005-10
7 He Shu-Mei, Liu Jing-Lan(Experiment central, Hebei Normal University, Shijiazhuang 050016);Study on the Determination Method of Flavone Content in Tea[J];Chinese Journal of Analytical Chemistry;2007-09
8 WU Fu-huo~(1),XIE Jin-dong~(2),XIE Xue-jian~(1),et al.(1.Department of Pharmacy,FCTCM,Fuzhou,Fujian 350003,China;2.Central Laboratory,FCTCM,Fuzhou,Fujian 350003,China);Study on Establishing Model of Hyperplasia of Prostate Gland in Rats[J];Journal of Fujian College of Traditional Chinese Medicine;2005-06
9 ZHOU Jian-heng1, HONG Zhen-feng1, LIN Jiu-mao2, et al (1. Department of Integrative Medicine, FUTCM, Fuzhou, Fujian 350108, China; 2. Fujian Academy of Integrative Medicine, FUTCM, Fuzhou, Fujian 350108, China);Effect of Qianliening Granule on Experimental Hyperplasia of Prostate[J];Journal of Fujian University of Traditional Chinese Medicine;2008-05
10 OU Min-rui~1, DAI Xiao-fu~1, XUE Feng-chun~1, ZHANG Yi~2, XU Xiao-ping~1 (1. College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, Fujian 350002, China; 2. Office of Educational Administration, Fuzhou University, Fuzhou, Fujian 350002, China);Analysis on the activity of 5α-reductase using high performance liquid chromatography[J];Journal of Fuzhou University(Natural Sciences Edtion);2004-02
【Secondary References】
Chinese Journal Full-text Database 7 Hits
1 CHAI Yi-feng*,ZHU Zhen-yu and CHEN Xiao-fei(Department of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University,Shanghai 200433),Fenxi Shiyanshi,2010,29(9):78~122;Pharmaceutical analysis(I)[J];Chinese Journal of Analysis Laboratory;2010-09
2 DANG Juan,YU Kai*,WANG Yao(Bioengineering College,Southwestern Jiaotong University,Chengdu,Sichuan,610031 P.R.China);Study on treatment of Pterocarpus indcus Willd extracts on rat androgenetic alopecia[J];West China Journal of Pharmaceutical Sciences;2010-03
3 WU Chuanling,MA Songtao(School of Pharmacy,Chengdu Medical College,Chengdu 610083,Sichuan,China);5α-reductase Inhibitors Screening Model in Vitro[J];Journal of Liaoning University of Traditional Chinese Medicine;2013-07
4 WU Jian-hui,SUN Zu-yue National Evaluation Center for the Toxicology of Fertility Regulating Drugs,Shanghai Institute of Planned Parenthood Research,Shanghai 200032,China;Establishment of an in vitro screening model for steroid 5 alpha-reductase inhibitors with the microplate reader[J];National Journal of Andrology;2013-06
5 Li Tao1, Li Yu-Hua2, Li Run-Sheng2, Gui Ya-Ping1, Bian Cui-Dong1, Huang Shen-Song1, Wu Deng-Long1 1Department of Urology ,Shanghai Tongji Hospital,Shanghai 200065 2Shanghai Institute of Planned Parenthood Research;Effects of epristeride on human prostate carcinoma cell Line DU-145[J];Surgical Research and New Technique;2012-03
6 Wu Chuanling,Ma Songtao,Wang Fuqin,Lan Xiaoping,Meng Lijuan(School of Pharmacy,Chengdu Medical College,Chengdu,Sichuan,China 610083);Study of Inhibiting Effect of Lotus Extract on 5α-Reductase[J];China Pharmaceuticals;2012-10
7 Zhou Benhong~(1,2),Guo Min~1,Wu Lining~2,Chen Dong~2(1.Department of Pharmacy,Renmin Hospital of Wuhan University,Wuhan 430060,China;2.School of Pharmaceutics Science,Wuhan University);UV-spectrophotometric Screening of 5α-Reductase Inhibitors in vitro[J];China Pharmacist;2011-12
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 LIU Su-huan,LI Zhe,ZUO Wen-jing,et al(Deptarment of Cell Biology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China);Changes of the Weight and Coefficient of Sexual Gland and the Level of Sexual Hormone in Adult Diabetic rat[J];Jilin Medical Journal;2002-05
2 Yu-Gui CUI1, Gui-Yuan ZHANG2 (1. Department of Endocrinology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China; 2. National Research Institute of Family Planning, Beijing 100081, China);Steroid 5α-Reductase Activity, Androgen Level and Its Receptor in the Target Tissues of Male Fetuses[J];Acto andrologica Sinica;2001-04
3 Wang Zhong Shan, et al Norman Bethune University of Medical Sciences(Changchun 130021);Observation on sexual organ weight and behavior in pubertal rats with diabetes[J];;1996-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved