Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Pharmacology and Clinics of Chinese Materia Medica》 2010-02
Add to Favorite Get Latest Update

Antihypertensive effect of puerarin in renal hypertensive rats and its effect on level of angiotensin II in renal tissue

Zhang Nianbao,Chen Huizhen,Cui Weidong,Ding Boping,Huang Zhenggui (Department of Pharmacology,Wannan Medical College & Pharmacology of TCM Grade Three Laboratory,State Administration of Traditional Chinese Medicine ,Wuhu 241002)  
Objective:This experiment was undertaken to observe effects of puerarin on blood pressure and level of angiotension II (ANG II) in renal tissue in two-kidney,one-clip (2K1C) hypertensive rats,and to investigate whether puerarin has an antihypertensive effect and a role in amelioration of renal interstitial fibrosis. Methods:70 Sprague-Dawley rats weighing 180 to 220 grams,half male and half female,were used. Renal hypertensive rat model was established by two-kidney,one-clip. High-,middle-and low-dose puerarin (200 mg/kg,100 mg/kg and 50 mg/kg,respectively) was given intraperitoneally for six weeks. In addition,a sham operation group was set and fed with normal diets. Each group had 8 rats. Blood pressure was measured every two weeks. Rats were sacrificed and kidneys were taken out after 6 weeks of treatment with puerarin. Contents of ANG Ⅱ in renal homogenate were measured by radioimmunoassay. Levels of renal interstitial fibrosis were observed via HE stained kidney slices. Results:High-,middle-and low-dose puerarin decreased blood pressure,and showed a dose-dependent effect (P0.01 or P0.05). Renal interstitial fibrosis was aggravated,which came along with an increase in the level of ANG Ⅱ in renal tissue. High-and middle-dose puerarin reduced levels of ANG II markedly (P0.01 or P0.05),while low-dose puerarin did not change the level of ANG II (P0.05). Puerarin at all three concentrations showed a role in amelioration of renal interstitial fibrosis (P0.01). Conclusion:Puerarin has a dose-dependent antihypertensive effect in renal hypertensive rats. It shows a role in the improvement on renal interstitial fibrosis,and the mechanism may be related to a decrease in level of ANG II in renal tissue and to its antihypertensive effect.
【Fund】: 安徽省自然科学基金资助(编号:070413124)
【CateGory Index】: R285.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved