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《Pharmacology and Clinics of Chinese Materia Medica》 2010-06
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Cytotoxicity effects of shikonin on rat cardiomyocytes in vitro

Gui Chun,Chen Menghua,Lin Song,Wei Yongxian(Department of Cardiology,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021)  
Objective: To test the toxicity effects and toxicity concentrations of shikonin on cultured rat cardiomyocytes.Methods: The neonatal rat ventricular myocytes were isolated and cultured,and treated with various concentrations of shikonin for the indicated time periods.then the drug toxicity of shikonin on cardiomyocytes was measured by MTT assay,trypan blue uptake assay,TUNEL and Hoechst staining.Furthermore,Western Blot was made to examine the changes of apoptosis passage protein,such as Bcl-2/Bax ratio and caspase3 activation.Results: There were no significant toxicity effects on cardiomyocytes when the concentration of shikonin was less than 1.3μM.but more than 1.3μM,shikonin appeared toxicity.The toxicity effects were dose dependent,from 1.3μM to 3μM,the higher were shikonin concentrations,the severer were toxicity effects.Cardiomyocytes were treated with 1.9μM and 2.5μM shikonin for 12 hours,the Bcl-2 expression of Cardiomyocytes was decreased significantly,and Bax expression of Cardiomyocytes was increased significantly,and Cleaved-caspase3 also was increased significantly.Conclusion: There are significant toxicity effects on cardiomyocytes when the concentrations of shikonin are more than 1.3μM,and the toxicity effects are dose dependent.Apoptosis mechanism contributes to the toxicity effects.
【CateGory Index】: R285
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