Naringenin attenuates acute lung injury induced by lipopolysaccharide in mice
Zhang Tianzhu;Ma Chunhua;Fan Xiangzhe;Changchun University of Chinese Medicine;China Pharmaceutical University;
Objective: determine the protective effect of naringenin on acute lung injury induced by lipopolysaccharide( LPS). Methods: The BABL / C mice were randomly allocated into control,LPS,Dex( 2mg / kg) + LPS group,naringenin( 20mg / kg) + LPS group,and naringenin( 40mg/kg) + LPS groups. LPS was intraperitoneally injected at a dose of 5mg/kg body weight in the LPS group,along with equal volumes of saline in the control group. Naringenin + LPS groups and Dex( 2mg / kg) + LPS group were treated with naringenin( 20mg / kg,40mg / kg,intraperitoneally) 1h prior to the injection of LPS or saline,respectively. The mice were killed 6h after LPS challenge. The bronchoalveolar lavage fluid( BALF) samples were analyzed for the concentration of numbers of neutrophilic、SOD、IL-6and TNF-α,and The lung samples were taken for histologic evaluation and for determination of wet-to-dry( W / D) lung weight,( MPO) level. Results: Pretreatment with naringenin prior to the administration of intratracheal LPS significantly induced a decrease in lung wet weight / dry weight ratio,in total leukocyte number and neutrophil percent in the BALF,and in MPO activity of lung in dose-dependent manners,and in IL-6,the tumor neurosis factor-α( TNF-α) in the BALF. At the same time,pretreatment with naringenin also significantly improved the superoxide dismutase( SOD) activity in the bronchoalveolar lavage fluid( BALF). These results showed that anti-inflammatory effects of naringenin against the LPS-induced ALI.