Determination of cholesterol content in edible oils and fats by gel permeation chromatography combined with high performance liquid chromatography-evaporative light scattering detector
WU Liping;ZENG Hui;SUN Yongmei;XIE Bingying;Panzhihua Product Quality Supervision and Inspection Institute;Panzhihua University;
A method for the determination of cholesterol content in edible oils and fats was established by gel permeation chromatography system combined with liquid chromatography-evaporative light scattering detector. The results showed that the linearity correlation was good in the range of 2-200 μg/m L with correlation efficient 0. 998 9. The limit of detection,recovery rate and relative standard deviation were10 mg/kg,94. 01%-103. 80% and 1. 12%-7. 25% respectively. The method could effectively reduce the loss of cholesterol caused by cumbersome pre-treatment process,and avoid solvent response and solvent peak interference during detection. The method was proved to be simple,feasible and effective.
【CateGory Index】： O657;TS227