Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Tumor》 2008-08
Add to Favorite Get Latest Update

Silencing PPARγ gene inhibits proliferation and inducs apoptosis of hepatoma HCCLM3 cells

WU Xu-dong1,CHEN Wei-chang2,HUANG Xiao-ping2(1.Department of Gastroenterology,First People's Hospital of Yancheng City,Yancheng 224001,China;2.Department of Gastroenterology,First Affiliated Hospital of Soochow University,Suzhou 215006,China)  
Objective: To observe the effects of knocking down the expression of peroxisome proliferators-activated receptor gamma(PPARγ) with RNA interference techniques on the proliferation and apoptosis of hepatocellular carcinoma HCCLM3 cells.Methods:A short-hairpin RNA(shRNA) eukaryotic expression vector against PPARγ was constructed and transfected into HCCLM3 cells.The changes of PPARγ expression were detected by semi-quantitative RT-PCR and Western blotting analysis.The proliferation of HCCLM3 cells was tested by MTT assay.Apoptosis ratio of HCCLM3 cells was detected by TUNEL method and flow cytometry(FCM).Expressions of PCNA and wide-type p53 protein were analyzed by immuocytochemistry(ICC) methods.Results:The sequence-specific shRNA(pshPPARγ) efficiently blocked the expression of PPARγ mRNA by 80.5%.At 48 h after transfection of pshPPARγ,proliferation of HCCLM3 cells was significantly suppressed by 71.5%.The positive rate of PCNA expression was(23.8±7.2)% at 40 h transfection.The apoptotic rates were(24.2±4.7) % as detected by TUNEL assay and(23.2±4.2)% of cells as measured by FCM test,respectively.The detection results of the two methods were consistent.In pshPPARγ transfection group,cell cycle of HCCLM3 cells was arrested in G0/G1 phase and the proportion of cells in G2/M phase decreased.Moreover,expression of wide-type p53 protein increased significantly.Conclusion:Knockdown PPARγ expression with RNA interference technology can significantly suppress proliferation and induce apoptosis of HCCLM3 cells.It is related with up-regulation of wide-type p53 protein expression.
【CateGory Index】: R735.7
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 ZHAO Ying-hai,HUANG Fang,JING Zhi-liang (Department of Pathology,Guangdong Medical College,Zhanjiang 524023,China);Expression of PPARγ,p-ERK,cyclin D1 and Ki-67 proteins and their implication in nasopharyngeal carcinoma cells[J];Chinese Journal of Clinical and Experimental Pathology;2010-02
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 CHEN Liangdong,LI Yan,ZHENG Fang,LI PengchengDept.of Oncology,Zhongnan Hospital of Wuhan University & Cancer Center of Wuhan University,Wuhan 430071,China;Study on the Intraperitoneal Chemotherapy with Hydroxycamptothecin for the Treatment of Peritoneal Carcinomatosis in Mice[J];Medical Journal of Wuhan University;2008-06
2 LEI Zhan-ping,ZHU Jian-si,WANG Cheng-kun,et al(Institute of Cancer Research,Nanhua University,Hengyang,Hunan 421001,China);The Identification and Screening of the High Invasive Metastatic Strain of Mice Histiocytosarcoma L-Ⅱ[J];Journal of Nanhua University(Medical Edition);2006-03
3 YANG Han-zheng,WU Xu-dong1,CHEN Wei-chang2(Institute of Immunology,Yancheng Health Vocational & Technical College,Yancheng 224006;1Department of Gastroenterology,the First People Hospital of Yancheng,Yancheng 224006;2Department of Gastroenterology,the First Affiliated Hospital of SooChow University,Suzhou 215006,China);PPARγ knockdown inhibited cell invasion and metastasis of HCCLM3 cells[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2008-07
4 WU Xu-dong1,CHEN Wei-chang1,ZHU Shu-yang2,YE Jian-xin1 1.Department of Gastroenterology,First affiliated Hospital of Soochow University,Suzhou 215006,P.R.China2.Department of Pediatrics,North Courtyard of First People's Hospital of Chenzhou City,Chenzhou 423000,P.R.China;Effect of RNA interference targeting at peroxisome proliferator-activated receptor gamma gene on angiogenesis of hepatocellular carcinoma in nude mice[J];Chinese Journal of Cancer Prevention and Treatment;2009-07
5 CHU Fang,YU Hua,FANG Lu,ZHOU Yan-yan,ZHENG Guo-an,FAN Qi-kun,DING Qi(Jiangxi Institute of Materia Medica,Nanchang 330029,China);A Comparative Study on the Liver and Spleen Metastasis of Three Transplanted Carcinomas in Nude Mice[J];Laboratory Animal Science;2012-06
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 He Xia, 2Min Huaqing, 2Lin Zhongning, 2Wu Qiuliang, 2He Jiehua, 2He Jinghui, 2Feng Kaitao ( 1Department of Radiation Oncology,Jiangsu Tumor Hospital, Nanjing 210009; 2Sun Yat sen University of Medical Scien;Correlation between factor of cell proliferation and prognosis in nasopharnygeal carcinoma(NPC)[J];Chinese Journal of Clinical and Experimental Patholog;2001-03
2 Xu Jin,Wen Jifang,Li Jinghe, Fu Chunyan (Department of Pathology, Xiangya School of Medicine,Central South University, Changsha 410078);Expression and significance of Smad_2 and p42~(ErK1)/p44~(ErK2) gene in gastric cancer[J];Chinese Journal of Clinical and Experimental Patholog;2002-02
3 He Lianzhi 1, Huang Wenbin 2, Zhang Ying 1, Zhang Zhengrong 1, Yang Junwen 1, Xu Guoxiang 3 ( 1Department of Obstetric and Gynecology, 3Department of Pathology, Yijishan Hospital, Wannan Medical College, Wuhu 241000; 2Department of Pathology, Wannan Medical College,Wuhu 241001);Expression of cyclinD1, PCNA and Ki-67 in endometrial adenocarcinoma and its significance[J];Chinese Journal of Clinical and Experimental Pathology;2003-06
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 ZHAO Meng,LIU Yue-ping,LIU Shi-zheng,WANG Xiao-ling (Department of Pathology,the Fourth Affiliated Hospital,Hebei Medical University,Shijiazhuang 050011,China);The role and significance of ERK and its activation in esophageal squamous cell carcinoma[J];Chinese Journal of Clinical and Experimental Pathology;2011-11
2 ZHAO Ying-hai,JING Zhi-liang,LI Fei-hong(Department of Pathology,Guangdong Medical College,Zhanjiang 524023,Guangdong,China);Relationship between epidermal growth factor receptor and the correlated-signal proteins in nasopharyngeal carcinoma and its effects on cell proliferation[J];Biomedical Engineering and Clinical Medicine;2011-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved