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《Tumor》 2008-10
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Effect of silencing PPARγ by RNA interference on growth of human gastric carcinoma cells

MA Xiu-mei,YU Hong,HUAI Na (Department of Pathology, Inner Mongolia Medical College, Huhht 0100590,China)  
Objective: To explore the role of peroxisome proliferator-activated receptor γ (PPARγ) expression in human gastric carcinoma using RNA interference technology. Methods: Three targeted locations were designed and three plasmid vectors (Y1, Y2 and Y3) expressing short hairpin RNA (shRNA) tartgeting PPARγ were constructed according to transcription RNA location of human PPARγ gene sequence and designing principle of small interference RNA (siRNA). Also a negative control plasmid vector HK was constructed. They were transfected into human gastric carcinoma MGC803 cells mediated by cationic liposome vector; the transfection efficiency of plasmid vector was evaluated by flow cytometry (FCM); PPARγ mRNA and protein expression levels were detected by RT-PCR and Western blotting, respectively. Proliferation of cells was measured by MTT assay and cell apoptosis was detected by contrast phase microscopy, Hoechst 33342 staining, and FCM, respectively. Results: Silencing PPARγ by RNA interference (RNAi) inhibited proliferation and induced apoptosis of human MGC803 cells. Conclusion:These results suggest that PPARγ over-expression suppressed the carcinogenesis of human gastric cancer through inhibiting proliferation and inducing apoptosis. PPARγ has the potential to become a new target for treatment of gastric carcinoma.
【CateGory Index】: R735.2
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 Guo Minglei, Li Jingjun, LI Hongnian, WAN Da fang, Gu Jianren. (Department of Biochemistry , Shanghai Cancer Institute,Shanghai 200032,China);Enhanced expression of peroxisome proliferator-activated receptor γ in liver cancer[J];Tumor;2005-05
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【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 Zhang Meng,He Hongtao,Zhou Ye,Xu Zhuo,Yang Tao,Liang Zhanqiang,Peng Li(Department of Hepatobiliary Surgery,Fourth Hospital,Hebei Medical University,Shijiazhuang,Hebei Province,050011,China);Expression and prognostic significance of PPAR-γ and PTEN protein in hepatocellular carcinoma[J];Journal of Third Military Medical University;2011-14
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7 WU Xu-dong1,CHEN Wei-chang2,HUANG Xiao-ping2(1.Department of Gastroenterology,First People's Hospital of Yancheng City,Yancheng 224001,China;2.Department of Gastroenterology,First Affiliated Hospital of Soochow University,Suzhou 215006,China);Silencing PPARγ gene inhibits proliferation and inducs apoptosis of hepatoma HCCLM3 cells[J];Tumor;2008-08
【Co-references】
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1 WANG Shu-sen, GUAN Zhong-zhen,XIANG Yan-qun, WANG Bo, LIN Tong-yu, JIANG Wen-qi, ZHANG Li, ZHANG Hui-zhong, HOU Jing-hui.Department of Medical Oncology, Cancer Center, Sun Yat-sen University, Guangzhou 510060, China;Significance of EGFR and p-ERK expression in nasopharyngeal carcinoma[J];Chinese Journal of Oncology;2006-01
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【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 Su Lianming,Ai Jiang,Kong Xianghong(Hongqi Hospital,Mudanjiang Medical College,Heilongjiang 157001,China);Expression of PTEN and its relationship with p-ERK in colorectal cancer[J];Chinese Journal of Histochemistry and Cytochemistry;2012-03
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