Effect of silencing PPARγ by RNA interference on growth of human gastric carcinoma cells
MA Xiu-mei,YU Hong,HUAI Na (Department of Pathology, Inner Mongolia Medical College, Huhht 0100590,China)
Objective: To explore the role of peroxisome proliferator-activated receptor γ (PPARγ) expression in human gastric carcinoma using RNA interference technology. Methods: Three targeted locations were designed and three plasmid vectors (Y1, Y2 and Y3) expressing short hairpin RNA (shRNA) tartgeting PPARγ were constructed according to transcription RNA location of human PPARγ gene sequence and designing principle of small interference RNA (siRNA). Also a negative control plasmid vector HK was constructed. They were transfected into human gastric carcinoma MGC803 cells mediated by cationic liposome vector; the transfection efficiency of plasmid vector was evaluated by flow cytometry (FCM); PPARγ mRNA and protein expression levels were detected by RT-PCR and Western blotting, respectively. Proliferation of cells was measured by MTT assay and cell apoptosis was detected by contrast phase microscopy, Hoechst 33342 staining, and FCM, respectively. Results: Silencing PPARγ by RNA interference (RNAi) inhibited proliferation and induced apoptosis of human MGC803 cells. Conclusion:These results suggest that PPARγ over-expression suppressed the carcinogenesis of human gastric cancer through inhibiting proliferation and inducing apoptosis. PPARγ has the potential to become a new target for treatment of gastric carcinoma.