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《Tumor》 2010-04
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Effects of exogenous fragile histidine triad gene on apoptosis of MGC-803 gastric cancer cells induced by octreotide

LUO Ling-li1,2,DUAN Xiao-ming2,WU Zheng-mao1,2,HE Xiu-sheng3 (1.Graduate School,University of South China,Hengyang 421001,China;2.Department of Digestive Medicine,Fourth Hospital of Changsha,Changsha 410004,China;3.Cancer Research Institute,University of South China,Hengyang 421001,China)  
Objective:To investigate the effect of exogenous extopic fragile hisdidine triad(FHIT) gene on the apoptosis of gastric cancer cells induced by octreotide and elucidate the underlying mechanism.Methods:Human gastric cancer cell line MGC-803,which was loss of FHIT gene,was transfected with plasmid pRcCMV-FHIT and pRcCMV via lipofectamine mediation.After transfection,Western blotting was used to analyse the expression of FHIT protein in positive cells screened out by G418.The cells were treated with octreotide 10-10,10-9,10-8,10-7,and 10-6 mol/L for 24,48 and 72 h.Cell proliferation was evaluated by MTT assay and apoptosis by flow cytometry.The expression of bcl-2 and bax protein was detected with Western blotting.Results:FHIT protein expression was detected in MGC-803 gastric cancer cells after FHIT gene transfection.After treatment with octreotide 10-8 mol/L for 48 h,the apoptotic rate of MGC-803 cells transfected with FHIT gene was(26.777±1.702)%,significantly higher than that in empty vector group and untransfected group [(13.800±0.511)% vs(12.634±0.479)%,F=245.789,P0.05].The expression of bcl-2 protein was decreased while the expression of bax protein was increased in FHIT gene transfection group after octreotide treatment.Conclusion:The exogenous FHIT gene and octreotide had strong synergistic effects on apoptosis of MGC-803 cell lines,and their action mechanism may be related to the alteration of the expression of apoptosis-related proteins of bcl-2 family.
【CateGory Index】: R735.2
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