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《CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE》 2000-09
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Effect of Ginsenosides on Proliferation and Differentiation of Human CD_(34)~+ Hematopoietic Stem/Progenitor Cells

JIN Jinmei, Tao Helen, GAO Ruilan, et al The Affiliated Hospital of Zhejiang College of TCM, Hangzhou (310006)  
To investigate the effect of ginsenosides (GS) on proliferation and differentiation of human CD 34 + stem/progenitor cells. Methods: CD 34 + hematopoietic progenitor cells were isolated from umbilical cord blood by using the immune beads sorting system. The cells were exposed to GS of different concentrations in both liquid culture and semi-solid culture, and the elevation rate on proliferation of CD 34 + stem/progenitor cells and colony formation were estimated. The cells were marked with monoclonal antibody and the marker was examined by flow cytometry after incubated with GS for 14 days. Results: GS (5-50μg/ml) could raise the colony production rate of BFU-E、CFU-E、CFU-GM、CFU-GEMM by (87 6±2 6)%、(63 3±2 8)%、(58 0±3 1)% and (96 3±5 5)% respectively (all P0 01), and the best effect in improving cell proliferation of CD 34 + cells in vitro was obtained when the concentration of GS was 25 μg/ml. After incubation with GS for 14 days, number of CD 33 + cells was increased by GS in a dose-dependent manner with a peak increasing rate at 200 μg/ml. In the presence of GS 50 μg/ml, CD 15 + cells were reaching the peak. Number of CD 71 +and G-A + cells increased only when the concentration of GS was 25 μg/ml. Conclusion: GS could not only promote the proliferation but also induce the differentiation of CD 34 + hematopoietic stem/progenitor cells, GS may play the role by cooperating with hematopoietic growth factor, and by its growth factor-like function in the regulation of hematopoiesis.
【Fund】: 中国和澳大利亚国际合作项目;; 澳方政府资助 教外司美 (1 996) 973号;; 国家中医药管理局资助!课题 (No .972 0 2 5);; 浙江省自然科
【CateGory Index】: R284
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