Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Integrated Traditional and Western Medicine》 2005-S1
Add to Favorite Get Latest Update

The Stimulation of Astragalus Membranaceus on Rats' Hepatic Oval Cell Growth Cultured in vitro

WANG Pin TANG Shu-zhen LIU Xiao-ming Hepatic Disease Center of Beijing Friendship Hospital,Capital Medical University,Beijing(100050)  
Objective To explore the effect of Astragalus Membranaceus(AM)on rats'hepatic oval cell growth cultured in vitro.Methods The rats were fed with 0.1% ethonine contained choline deficient diet for 4—6 weeks.Hepatic oval cells were isolated from rats'liver.Reverse transcription-polymerase chain reaction(RT- PCR),immunohistology,Western blot method were used to determine the cultured cells.Effect of AM in different concentrations on hepatic oval cell growth was observed by MTT method.Changes of cell phenotype and cell cycle were respectively observed by Giemsa staining and fluorescence activated cell sorter(FACS).Results The isola- ted cells not only expressed stem cell marker c-kit,but also expressed embryonic hepatic cell markerα-fetoprotein, cholangiocyte marker OV6 and CK19,as well as mature hepatic cell marker albumin,which were thought to be rats' hepatic oval cells.AM from the concentration of 0.125 mg/ml to 4 mg/ml had the stimulating effect on the growth of hepatic oval cells at 48 hrs,among which AM of 2 mg/ml had the most obvious stimulating growth effect (P<0.05).Some proliferation enhanced cells appeared treated by AM of 2 mg/ml 48 hrs later.So did some cells of lower nucleoplasmie ratio.The cell cycle determination showed that cells treated with AM in G2-S and M phase were increased more than the control cells without treatment by AM.Conclusion AM has obvious stimulating effect on the growth of hepatic oval cells cultured in vitro.
【Fund】: “肝脏保护与再生调节”北京市重点实验室开放课题资助
【CateGory Index】: R285
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 2 Hits
1 WANG Ping,YAN Zhong-yu,CONG Min,TANG Shu-zhen,CHEN Yi-yang, DONG Zhong,YOU Hong,JIA Ji-dong,WANG Bao-en (Liver Research Center,Beijing Friendship Honpital,affiliate of Capital University of Medical Sciences,Beijing,100050,China);The effects of sodium butyrate on the expression α-fetal protein(AFP)of hepatic oval cells[J];Journal of Clinical and Experimental Medicine;2004-02
2 Gao Guanyue; Ying Zizong;Fong Yishu et al(Department Biochemical, Worker's College of Wuhan,Wuhan 430016);Protecting Liver Effect of Radix Amgelicae Sinensis and Scutellaria Baicalensis[J];CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE ON GASTRO-SPLEEN;1997-04
Chinese Journal Full-text Database 10 Hits
1 WANG Yan-ping1,CHEN Yan2(1.Department of Chemistry and Chemical Engineering,Hefei Normal University,Hefei 230061,China; 2.Anhui Institute of Gedogical Experiment,Hefei 230001,China);Four Aza-crown Ether Complexes of Copper Affect on the Level of Carbon Clearance of Immunosuppressed Mice[J];Journal of Hefei Normal University;2011-06
2 TANG Chun-yi et al (College of Biological Resources and Environmental Science, Jishou University, Zhangjiajie, Hunan 427000);Dynamic Changes of the Total Flavone Content in Ophiopogon japonicus Fruit and its Extraction by a Microwave Technology[J];Journal of Anhui Agricultural Sciences;2005-01
3 CHEN Li-jun et al (Xinyang Agricultural College,Xinyang,Henan 464000);Preliminary Study on Antifungal Activity of the Extract from 7 Medicinal Plants[J];Journal of Anhui Agricultural Sciences;2006-21
4 WANG Gao-xue et al(College of Animal Science and Technology,Northwest A&F University,Yanglin,Shaanxi 712100);Effect of Oviductus Ranae Compound on mRNA Expression of α-actin inRat Skeletal Muscle[J];Journal of Anhui Agricultural Sciences;2007-11
5 ZHANG Yu et al (College of Life Science and Engineering,Southwest Jiaotong University,Chengdu,Sichuan 610031);Studies on Chemical Constituents in Volatile Oils of Tibetan Rhododendron lepidotum Wall. ex G. Don[J];Journal of Anhui Agricultural Sciences;2010-25
6 YAN Fu sheng,XIE Xiao yun,HUANG Xiao qiao,HUANG Hua jiu,XU Yuan xian, ZHU Xiao ling,LIU Gui hua,XU Ai lan,LIU Zhi hong,DONG Yu hua,LIN Jing e (Hanchuan Women & Children Hospital of Hubei Province,Hubei Hanchuan 432300,China);Clinical and Experimental Studies on Assisted Application of Qinggong Decoction in Drug-induced Artificial Abortion Technique[J];Journal of Anhui Traditional Chinese Medical College;2003-05
7 CHEN Hua,XIN Guang,ZHANG Lan-jie,ZHANG Bo (Department of Chemistry,Anshan Normal University,Anshan Liaoning 114005,China);Determination of Chemical Constituents of Essential Oil from Myrrha by GC-MS[J];Journal of Anshan Normal University;2006-02
8 LIU Fang(Medical science Department,Medical science academy of higher education,Baicheng 137000,China);Research on Anti-Fungal Activity of Essential Oil from Artemisiae Annuae[J];Journal of Baicheng Normal College;2009-06
9 Lu Liangwei; Zhang Ping ; Xiong Yaokang; et al(Zhejiang College of TCM, Hangzhou 310009);Experimental Study about Huoxue Shenshi Decoction's Anti-fibrosis in Liver[J];Journal of Zhejiang College of Tcm;1999-04
10 Lin Hong,Zhang Guoliang Anhui College of Traditional Chinese Medicine,Hefei (230038);Effects of Traditional Chinese Medicine Ruanganyin on TGF-1/Smad Signaling Pathway in Carbon Tetrachloride-induced Hepatic Fibrosis of Rats[J];Journal of Zhejiang University of Traditional Chinese Medicine;2008-02
China Proceedings of conference Full-text Database 3 Hits
1 TIAN Peng~(1,2),TAO Wen-hong~1,DU Yi-li~1,CAO Yong-zheng~1,LI Ke-ji~1,YANG Jin-xiu~1 (1.Department of Ultrasound,Affiliated Hospital of Zunyi Medical College,563003; 2.Department of Ultrasound.Daqing Oil Field General Hospital,163003);Application of ultrasound-guided free-hand biopsy in hepatic fibrosis[A];[C];2010
2 Chen Qing Li Jian Huang Yifan~* (College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou 350002);The effect of QiLing medicament on protein content and the activity of ALT,AST in serum of in the immunodepressed mice[A];[C];2008
3 Hua Haiying~1 Li Yanying~2 Ge Shiwen~1 (~1Zhengzhou University Academy of Medical and Pharmaceutical Sciences,Zhengzhou 450052;~2 Department of Pharmacy,Sanmenxia Huanghe Hospital,Sanmenxia 472000);Study of tetramethylpyrazine in treatment on liver fibrosis and mechanism in rats[A];[C];2007
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved