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《Chinese Journal of Integrated Traditional and Western Medicine》 2005-S1
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The Stimulation of Astragalus Membranaceus on Rats' Hepatic Oval Cell Growth Cultured in vitro

WANG Pin TANG Shu-zhen LIU Xiao-ming Hepatic Disease Center of Beijing Friendship Hospital,Capital Medical University,Beijing(100050)  
Objective To explore the effect of Astragalus Membranaceus(AM)on rats'hepatic oval cell growth cultured in vitro.Methods The rats were fed with 0.1% ethonine contained choline deficient diet for 4—6 weeks.Hepatic oval cells were isolated from rats'liver.Reverse transcription-polymerase chain reaction(RT- PCR),immunohistology,Western blot method were used to determine the cultured cells.Effect of AM in different concentrations on hepatic oval cell growth was observed by MTT method.Changes of cell phenotype and cell cycle were respectively observed by Giemsa staining and fluorescence activated cell sorter(FACS).Results The isola- ted cells not only expressed stem cell marker c-kit,but also expressed embryonic hepatic cell markerα-fetoprotein, cholangiocyte marker OV6 and CK19,as well as mature hepatic cell marker albumin,which were thought to be rats' hepatic oval cells.AM from the concentration of 0.125 mg/ml to 4 mg/ml had the stimulating effect on the growth of hepatic oval cells at 48 hrs,among which AM of 2 mg/ml had the most obvious stimulating growth effect (P<0.05).Some proliferation enhanced cells appeared treated by AM of 2 mg/ml 48 hrs later.So did some cells of lower nucleoplasmie ratio.The cell cycle determination showed that cells treated with AM in G2-S and M phase were increased more than the control cells without treatment by AM.Conclusion AM has obvious stimulating effect on the growth of hepatic oval cells cultured in vitro.
【Fund】: “肝脏保护与再生调节”北京市重点实验室开放课题资助
【CateGory Index】: R285
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