Study on the Apoptosis Inducing Effect of Pseudolaric Acid B on Cervical Carcinoma Cell Line HeLa Cells

HU Yun,WU Xiao-ke and HOU Li-hui Department of Obstetrics and Gynecology,the 211 Hospital of PLA,Harbin (150080)  

Objective To investigate the biological effect and mechanism of action of pseudolaric acid B (PAB)on the cultured human HeLa cells in vitro.Methods MTT and flow cytometric assays were used to detect the cells proliferation inhibitory rate and apoptosis of HeLa cell in exposure to PAB;the morphological changes of apoptosis were observed with electron microscope;and the expressions of p53/bcl-2/bax mRNA were detected by RT-PCR.Results(1)HeLa cell proliferation was inhibited by PAB in a dose-dependent manner,the IC_(50)being about 10μmol/L;(2)flow cytometry showed that the distribution of HeLa cell cycle was changed timedependently by 10μmol/L PAB-treatment,showing decrease of G_0/G_1 phase cell percentage and increase of G_2/M phase cell percentage;(3)the bax mRNA expression elevated and bcl-2 protein expression decreased markedly after being treated by 10μmol/L PAB for 12 h,24 h,and 48 h;while the expression of p53 mRNA could not be detected.Conclusion PAB can inhibit the proliferation and induce the apoptosis of Hela cells in vitro,and its molecular mechanism may be associated with up-regulating bax mRNA expression and down-regulating bcl-2 mRNA expression.

【Key Words】： pseudolaric acid B HeLa cervical cancer cell apoptosis
【Fund】： 黑龙江省自然科学基金项目(No:AD200647)
【CateGory Index】： R285.5;R737.33
【DOI】： CNKI:SUN:ZZXJ.0.2010-07-018

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