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《Shandong Agricultural Sciences》 2011-01
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Preparation and Identification of Monoclonal Antibody of Avian Infectious Bronchitis Virus

JIANG Yi-fei,QI Li-hong,HUANG Bing,LI Yu-feng,YU Ke-xiang,SONG Min-xun*(Poultry Institute of Shandong Academy of Agricultural Sciences/ Shandong Key Laboratory of Avian Disease Diagnosis and Immunization,Jinan 250023,China)  
BALB/c mice were regularly immunized with purified avian infectious bronchitis virus(IBV).Their spleen cells were collected and infused with myeloma cell Ag8.653.After selection in time and 3 times clone by limiting dilution,3 hybridoma cell lines against IBV named 1F4F7D9,3E9G3D4 and 4B8B3F8 were obtained.The indirect ELISA titers of their ascites were all above 106 by indirect ELISA detection,hemagglutination inhibition and agar gel precipitin test.It was proved that 3 cell lines all possessed the favourable specificity.
【Fund】: 国家科技支撑计划(2006BAD06A11);; 山东省农业科学院青年基金(2007YQN025)
【CateGory Index】: S852.65
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