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《Shandong Agricultural Sciences》 2011-06
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Effect of Recombinant Saccharomyces cerevisiae Expressing Salmon Calcitonin on Osteoclast Culture in vitro

JIANG Yong1,ZHANG Hui2,WANG Peng3,ZHAO Xi-xi1,XU Ke-feng1, WANG Jian1,ZHANG Xue-cheng4*(1.National Oceanographic Center in Qingdao,Qingdao 266071,China;2.China Rural Technology Development Center, Beijing 100045,China;3.Shandong Academy of Agricultural Sciences,Jinan 250100,China; 4.Ocean University of China,Qingdao 266003,China)  
The osteoclasts of new born Wistar mice were obtained by mechanical separation.The recombinant Saccharomyces cerevisiae expressing salmon calcitonin and restricted by enterokinase and Miacalcic were added into the culture solution respectively.The osteoclasts were observed by HE staining.After cultured for seven days,the area of bone resorption pits was analyzed by Image-Pro Plus image software.The results showed that the recombinant Saccharomyces cerevisiae expressing salmon calcitonin had obvious inhibitory action to the absorption function of osteoclasts.
【Fund】: 国家“863”高技术发展与研究计划项目(819-04-10)资助
【CateGory Index】: S917.4
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