Screening of High Acid - stable α - amylase Producing Strains by UV
You Wenjuan ( Hunan Chemical Industry Vocational Technology Institute,Zhuzhou 412004,China)
The UV mutagenesis influenced on acid - stable α - amylase yield from the producing strain Bacillus subtilis BF7658 has been studied. The results showed that the optimal conditions was irradiated 90s with 30W UV. A high and stable yield mutant was achieved by Screened with discolored zone and fermented in shake flask,and named UN - 12. The acid - stable α - amylase activity of UN - 12 was 3418. 8 U/mL,which increased 59. 7% compared with the original strain. The α -amylase activity of UN -12 increased a little by secondary UV treatment.
【CateGory Index】： TQ925