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《Anhui Agricultural Science Bulletin》 2018-11
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Cloning of Human Growth Differentiation Factor 15 Gene and Construction of Recombinant Prokaryotic Expression Vector

Dong Yanmin;College of Life Science,Jilin Agricultural University;  
For gene clone growth differentiation factor 15,constructing h GDF 15 gene recombinant prokaryotic expression vector,which laid the foundation for the study of the function of this gene,cloning thegene sequences using the PCR technology. It's connection to p ET28 a and p Cold I carrier respectively,construct recombinant p ET28 a h GDF15 and p Cold I h GDF15 prokaryotic expression vector,and verified plas-mid PCR and double enzyme digestion and sequencing.The results show that the method of recombinant carrier is successful,which lays a foundation for further research on the mechanism of h GDF15 protein.
【Fund】: 吉林省教育厅“十三五”科学技术项(2016-177)
【CateGory Index】: R587.1
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