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《Journal of Anhui Agricultural Sciences》 2006-17
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Establishment of the Detection of the Antibody against Follicular-stimulating Hormone with the Indirect ELISA

LI Hong-fei et al (Key Laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology,Ministry of Agriculture,College of Animal Science and Technology,Northwest A & F University,Yangling,Shaanxi 712100)  
An indirect enzyme-linked immunosorbent assay(ELISA) was standardized to detect the antibody against follicular-stimulating hormone(FSH).Being tested with the indirect ELISA,5 serum samples which were acquired by the FSH-BSA immunized BaLb/cmouses were all positive.The optimum work concentration of the indirect ELISA was established.Polystyrene micro-titer plates were coated by the purified FSH at theconcentration of 10?μg/ml.The working concentration of serum samples was 1∶500 and the sheep-against-mice IgG labeled by HRP was 1∶1?200.The confining liquid was 1.0% defatted milk powder.Antigenblockage test was designed to check the results.
【Fund】: 国家自然科学基金资助项目(39770545);; 西北农林科技大学青年骨干教师支持计划项目(01140305)
【CateGory Index】: Q503
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