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《Journal of Anhui Agricultural Sciences》 2006-17
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Establishment of the Detection of the Antibody against Follicular-stimulating Hormone with the Indirect ELISA

LI Hong-fei et al (Key Laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology,Ministry of Agriculture,College of Animal Science and Technology,Northwest A & F University,Yangling,Shaanxi 712100)  
An indirect enzyme-linked immunosorbent assay(ELISA) was standardized to detect the antibody against follicular-stimulating hormone(FSH).Being tested with the indirect ELISA,5 serum samples which were acquired by the FSH-BSA immunized BaLb/cmouses were all positive.The optimum work concentration of the indirect ELISA was established.Polystyrene micro-titer plates were coated by the purified FSH at theconcentration of 10?μg/ml.The working concentration of serum samples was 1∶500 and the sheep-against-mice IgG labeled by HRP was 1∶1?200.The confining liquid was 1.0% defatted milk powder.Antigenblockage test was designed to check the results.
【Fund】: 国家自然科学基金资助项目(39770545);; 西北农林科技大学青年骨干教师支持计划项目(01140305)
【CateGory Index】: Q503
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 WANG Xue-peng~1, LI Jian-liang~1, CHANG Wei-shan~1, LIU Yue-zhu~2, ZHANG Chun-lan~2(1. College of Animal Science and Technology, Shandong Agricultural University, Tai’an 271018,China; 2. Shandong Mingfa Veterinary Medicine Limited Company, Jinan 250000,China);Study on detection of the antibody against fowlpox virus by the indirect ELISA[J];Chinese Journal of Veterinary Science and Technology;2003-11
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Sheng-juan et al(Beijing University,Beijing 100871);Analysis and Improvement of Protein Detection Test by Double Antibodies Sandwich[J];Journal of Anhui Agricultural Sciences;2010-29
2 HE Lang~1 WANG Dan~1 YANG Yao-qin~2 (1.Department of Biochemistry,Chengdu Medical College,Chengdu 610083 China; 2.Tumor Research Center of Tongji University,Shanghai 200091 China);Preparation and Characterization of Monoclonal Antibody Against Human Idiopathic Gallbladder Carcinoma[J];Journal of Chengdu Medical College;2006-01
3 JING Hong-li,LI Qiang,WU Qiu-xian,LI Hua(Key Laboratory of Mariculture,Agriculture Ministry,PRC,Dalian Ocean University,Dalian 116023,China);Establishment of monoclonal antibody mediated indirect ELISA for detection of Shewanella smarisflavi[J];Journal of Dalian Ocean University;2010-06
4 ZHANG Feng1,2,ZHANG Li1,NI Hui1,ZHANG Si1,(1.Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province,Dalian Ocean University,Dalian 116023,China;2.School of Life Science and Technology,Dalian Ocean University,Dalian 116023,China);Detection of CD35 in coelomocytes of sea cucumber Apostichopus japonicus by chemiluminescence immunoassay[J];Journal of Dalian Ocean University;2011-02
5 MA Yun-zhi1,LI Xiao-yan2,WANG Xing-long2(1.Department of Agronomy,Jilin University,Changchun,Jilin,130062,China;2.Institute of Military Veterinary Medicine,Academy of Military Medicine PLA,Changchun,Jilin,130062,China);Establishment of Monoclonal Antibody Latex Agglutination Test for Detecting B.abortus[J];Progress in Veterinary Medicine;2008-02
6 Che Xiao-qing,Guo Ming* (Department ofChemistry,ZheJiangForestryUniversity,Linan,Zhejiang311300,China);Recent Progress of Enzyme-linked Immunosorbent Assay Technique for Heavy Metal Residues[J];Fujian Analysis & Testing;2009-04
7 SHANG Wei-hui1,LI Yong-tao1,SHU Wei2,M.Benedetti3(1.School of Natural Resource and Environment,South China Agriculture University,Guangzhou510642,China;2.Instrumental Analysis and Research Centre,South China Agriculture University,Guangzhou510642,China;3.Laboratoire de Geochimie des Eaux,Universite Paris-Diderot-IPGP,case7052,5 rue Thomas Mann,75205 Paris Cedex 13,France);Mechanism of Biological Indication and Catalytic Degradation of Cytochrome P450 to Contaminants[J];Environmental Science & Technology;2009-06
8 LUO Xinzheng1,2(1,Fujian Cadre School of Quality and Technology Supervise,Fuzhou 350002,Fujian,China)(2,Food Science College of Fujian Agriclture and Forestry University,Fuzhou 350002,Fujian,China);Common Heavy Metal Pollution in Food and Analysis Technologies[J];Quality and Technical Supervision Research;2010-06
9 Shao Jun,You Xiaogang,Gao Feng~,He Rong,Cui Daxiang(National Key Laboratory of Nano/Micro Fabrication Technology,Key laboratory for Thin Film andMicrofabrication of Ministry of Education,Institute of Micro and Nano Science and Technology,Shanghai Jiaotong University,Shanghai 200030);Labeling of Quantum Dots with Streptavidin and Its Bioactivity Measurement[J];Chinese Journal of Analytical Chemistry;2006-11
10 Han Dan,Yu Meng,Wu Mei,Deng An-Ping(College of Chemistry,Sichuan University,Chengdu 610064);A Sensitive Enzyme Linked Immunosorbent Assay for the Analysis of Sudan Ⅰ in Food Samples[J];Chinese Journal of Analytical Chemistry;2007-08
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG LinLujiang People's Hospital,Lujiang 231500,Anhui;Analysis of drug resistance of proteus and acinetobacter[J];Journal of Anhui Health Vocational & Technical College;2006-04
2 WANG Yongcheng\ CHANG Wenbao (The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering,Peking University,Beijing,100871) GUO\ Zhenquan (Life Sciences Center,Peking University,Beijing,100871);Production and Characterization of Anti-estriol Monoclonal Antibody[J];Acta Scicentiarum Naturalum Universitis Pekinesis;2002-04
3 ZHU Qiao-ling(Department of Laboratory,Third People's Hospital of Chengdu City,Chengdu 610000,China);Analysis of drug resistance of Proteus mirabilis to commonly used antibiotics[J];Journal of Chengdu Medical College;2009-04
4 WANG Hong-lei1,GUO Wen-long2(1.Changqing VeterinaryBureau jinan 250300;2.Shandong Agricultural University Animal Science College);Manufacture and Bionomics determine of Subunit Vaccine in Avian Proteus mirabilis[J];Poultry Science;2009-03
5 GUO Xiao-hua,ZHAO Zhong-xin*(Department of Neurology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China);Regulation mechanism of unfolded protein response: progress and clinical prospect[J];Academic Journal of Second Military Medical University;2007-03
6 ZHANG Zhi,ZHAO Hong kun,CUI Zhi zhong (Animal Science and Technology of Shandong Agriculture University,Taian,Shandong,271018,China);Investigation on Subgroup J avian leukosis virus in Parts of China[J];Progress In Veterinary Medicine;2003-05
7 Wang Congzhu, Wang Hong Laboratory of Medical Technology, Shanghai Second Medical University (200023);Preparation and Characterization of Monoclonal Antibodies Against Thyroid Stimulating Hormone (TSH)[J];Journal of Radioimmunology;2005-02
8 SHI Fu-shan,ZHAO De-ming(Laboratory of National Animal TSE,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China);Isolation and Identification of 16S rRNA of Escherichia coli from Chicken[J];China Animal Husbandry & Veterinary Medicine;2009-02
9 Huang Yinyao; Kong Fande; Zhou Binhua(Xiamen Animal and Plant Quarantine Bureau)Chao Guojin; zhuanYaozhong; Lai Qinjin(Changtai operating pig farm);Report of Sow Abortion andDeath Caused by E. Colitogether with Proteus[J];FUJIAN JOURNAL OF ANIMAL HUSBANDRY;1996-01
10 JIN Ting,GUAN Yixin,FEI Zhengzheng and YAO Shanjing (Department of Chemical and Biochemical Engineering,Zhejiang University, Hangzhou 310027,Zhejiang,China);RENATURATION OF RECOMBINANT HUMAN INTERFERON-γ INCLUSION BODY BY DILUTION[J];Journal of Chemical Industry and Engineering(China);2004-05
【Secondary References】
Chinese Journal Full-text Database 5 Hits
1 ZHOU Fang;RAN Dan-dan;LIU Fei;TANG Cheng;YUE Hua;College of Life Science and Technology,Southwest University for Nationalities;Veterinary Medicine Key Laboratory of Sichuan Institutes of Higher Learning;;Isolation and Identification of Proteus mirabilis from Chickens and Analysis of Phylogenetis[J];Progress in Veterinary Medicine;2015-07
2 WU Hong-chao;LIU Xiao-ying;FENG Er-kai;ZHU Yan-zhu;WAN Hong-li;FENG Zhuo;YAN Xi-jun;CHEN Li-zhi;Institute of Special Animal and Plant Sciences,Chinese Academy of Agricultural Sciences;Jilin Teyan Biological Technology Limited Liability Company;;Prokaryotic Expression and Immunogenicity Analysis of Hemagglutinin-related Outer Membrane Protein fromF.necrophorum[J];China Animal Husbandry & Veterinary Medicine;2015-11
3 Su Weiyi;Li Xinnan;Han Juanzhu;Liaoning province of veterinary feed quality and safety of animal products testing center;;Drug-resistance research and elimination of drug resistance plasmids of 10 strains of chicken source Proteus mirabilis[J];Modern Journal of Animal Husbandry and Veterinary Medicine;2014-10
4 LI Xin-nan;HAN Juan-zhu;NING Yi-bao;Liaoning Provincial Animal Feed Quality and Safety of Veterinary Testing Centers;China Institute of Veterinary Drug Control;;Isolation and identification of Proteus mirabilis from chicken and study of its drug resistance[J];Heilongjiang Animal Science and Veterinary Medicine;2015-11
5 CUI Guo-lin,ZHONG Shi-xun,YANG Shi-fa,ZUO Xue-mei,ZHU Rui-liang*(Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology,Shandong Agricultural University,Taian 271018,China);Isolation and Identification of Proteus mirabilis from Goat and the Analysis of Its 16S-23S rRNA ISR Sequence by RFLP[J];Chinese Journal of Animal and Veterinary Sciences;2013-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WU Guo juan 1 SHEN Hong 1 ZHANG Zhong wen 1 ZHOU De gang 2 et al ( 1Beijing Agricultural College,Beijing 102206; 2Beijing Institute of Veterinary Drug Control,Beijing 100101);Assay on Residue of Antibiotics in Sterilized Pure Milk from Market[J];Journal of Beijing Agricultural College;2002-02
2 BAISHANBO Asiya, GU Li-Su*, HU Chang-Qin*, TAN Huan-Ran(Department of Pharmacology, School of Basic Medical Sciences, Beijing Medical University,Beijing, 100083) *(Department of Antibiotic, National Institute For The Control of Pharmaceutical and Biologic;STUDY ON THE RELATIONSHIP BETWEEN ANTI-STREPTOMYCIN ANTIBODY AND THE EAR-TOXICITY AND THE NEPHROTOXICITY[J];THE CHINESE JOURNAL OF CLINICAL PHARMACOLOGY;1999-05
3 Xie Nanping, Wang Liwen, Wang Boyang (Second Affiliated Hospital of First Military Medical University, Guangzhou);Ultrastructural observation on streptomycin-induced defect of the inner ear vestibule in guinea pigs[J];Journal of Clinical Otorhinolaryngology;1993-01
4 HE Shu-xiang (Laboratory of third Hospital of Chifeng,Inner Mongolia 024001 China);The Change and Mechanism on Resistance of the Staphylococcus aureus[J];Nei Moivgol Medical Journal;2001-04
5 Li Youye(Shenyan University);Streptomcin at elevated doses with acute bronchopneumonia in piglets[J];VETERINARY PHAACEUTICALS & FEED ADDITIVES;1999-05
6 Kong Lingxing(kong LX)(Pharmacy,Beiing Medical University,People′s Hospital,Beijing 100044);Determination of potency of low concentration streptomycin sulfate in examination injection by rotatory method[J];CHINESE JOURNAL OF MODERN APPLIED PHARMACY;2000-02
7 Bai Li ,Zhao Wen-feng ,Li Mei-rong ,et al(Pingdingshan Institute for Drug Control 467000);Determination of streptomycin sulfate with a concentrate solution[J];CHINESE JOURNAL OF HOSPITAL PHARMACY;1995-01
8 LI Shuhua, HAN Dongyi, YANG Weiyan, et al. *?Department of Otorhinolaryngology, Shenyang General Hospital of PLA, Shenyang 110015, China Corresponding author:LI Shuhua (Email: shuhua -lee@263.net);The effects of streptomycin on the calcium influx in SGCs during depolarization[J];;2001-02
9 Hu Chang-qin, Zhao Jian-xi and Jin Shao-hong (National Institute for the Control of Pharmaceuticals and Biological Products, Beijing);CHANGES OF PROTEIN CONFORIVIATION BY STREPTOMYCIN INTERACTING WITH PROTEIN[J];Chinese Journal of Antibiotics;1990-01
10 Zhou Yu Kong Ying-mei Tang Qiu-jin and Zheng Chang-Hang (National Institute for the Control of Pharmaceutical and Biological Products, Beijing);SEPARATION AND DETERMINATION OF STREPTOMYCIN BY REVERSE PHASE HPLC[J];Chinese Journal of Antibiotics;1990-03
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