Establishment of Tissue culture and Rapid Propagation System in Ficus elastica
ZHAO Hai-qing et al (Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Science,Urumqi,Xinjiang 830011)
[Objective] The aim of the research was to breed Ficus elastica by using tissue culture and rapid propagation technologies and meet the demands of flower market to this plant.[Method] With shoot tip of Ficus elastica as explants,different medium and hormone combinations were selected to set up the tissue culture and rapid propagation system of F.elastica preliminarily and make a comparative test.[Result] The medium for callus induction and bud differentiation was MS+1.0 mg/L 6-BA+0.01 mg/L NAA,the medium for the propagation of clumpy buds was MS+2.0 mg/L 6-BA+0.01 mg/L NAA and the optimum rooting medium was 1/2 MS+0.01 mg/L NAA+0.5 mg/L IBA.[Conclusion] This research provided practical basis for mass production of F.elastica.But the induction and germination technologies of somatic embryo still need to be improved.