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《Journal of Anhui Agricultural Sciences》 2012-15
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Establishment of ELISA for Detecting Protein SAG3 Antibody of RH Strain of Toxoplasma gondii

MA Liang et al(College of Animal Science and Veterinary Medicine,Jilin University,Changchun,Jilin 130062)  
[Objective] The research aimed to establish the indirect ELISA for detecting the purified surface protein SAG3 antibody of Toxoplasma gondii(T.gondii).[Method] Using chessboard titration method,the best conditions of indirect ELISA were determined.Taking negative serum as control,the positive serums of mouse against Neospora caninum,G.lamblia,C.andersoni,E.tenella and C.parvum were used to detect if the indirect ELISA has cross reactions with them.[Result] The optimal coating conditions were as follows:antigen coating concentration was 0.25 μg/hole,and the optimal dilution of the detected serum of T.gondii and enzyme-labeled second antibody were 1∶400 and 1∶3 000 respectively,blocking agent was the PBST solution of 5% nonfat-dry milk.The developed indirect ELISA had no cross reaction with positive serums.The sensitivity of this method was 1∶1 600.[Conclusion] This method had high sentivity,stronger specificity and better repeatability,which might be used for the diagnosis and epidemiological investigation of T.gondii with SAG3 protein.
【Fund】: 国家科技支撑计划课题(2008BAD96B11-3)
【CateGory Index】: S852.7
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