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《JOURNAL OF ANHUI NORMAL UNIVERSITY(NATURAL SCIENCE)》 1999-03
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RANDOM AMPLIFIED POLYMORPHIC DNA(RAPD) ANALYSIS OF FIVE BREEDS OF DOMESTIC CHINESE GOOSE

HAO Jia sheng 1,\ ZHOU Kai ya 2 (1.Department of Biology, Anhui Normal University, 241000, Wuhu, Anhui, China;2. Biodiversity and Molecular Evolution Laboratory, Nanjing Normal University, 210097, Nanjing, China)  
subjectives Random amplified polymorphic DNA(RAPD) analysis was used to assess genetic variability in five breeds of domestic Chinese goose(Wanxi white  breed, Taihu breed, Zhedong white breed, Sichuan white breed and Shitou breed). methods The total genomic DNAs of the five breeds of domestic Chinese goose were amplified through single primer PCR with 14 selected arbitary 10 oligonucleotide primers out of the 34 primers. results 14 primers give rise to 174 reproducible DNA fragments altogether in all the five breeds. 48 which accounts for 27.6% of the fragments are polymorphic, while the rest 126 fragments are monomorphic among all the species. Genetic distance coefficients between all the species were caculated according to the homogenity and diversity of the RAPD fragments, the mean coefficients between Wanxi white breed, with Taihu breed, Zhedong white breed, Sichuan white breed and Shitou breed was 0.1208,0.1571,0.1911,0.1805 respectively, those between Taihu breed, with Zhedong white breed, Sichuan white breed and Shitou breed was 0.1614,0.1712,0.1954 respectively, those between Zhedong white breed, with Sichuan white breed and Shitou breed was 0.1941,0.2016 respectively, that between Sichuan white breed and Shitou breed was 0.2274. The cluster analyses which derived form their mean genetic distance coefficients were performed with UPGMA and NJ methods using MEGA software. conclusions The genetic differentiations between the five breeds of domestic Chinese goose may be closely related to artificial selections but not to their geographical distances.
【Fund】: 高等学校博士点基金资助课题
【CateGory Index】: Q953
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