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《Journal of Anhui Normal University(Natural Science)》 2009-04
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Study on the Preparation and Properties of Supperoxide Dismutase from the Eudothelial of Newhall Orange

SHI Mei,ZHENG Jian-xin,LV Dong(College of Life Sciences,Anhui Normal University,Wuhu 241000,China)  
The organic solvent graduation precipitation method was used to separate SOD from inner layer of newhall orange.The result indicated:when the inner layer(g) of newhall orangeand phosphoric acid buffer solution(ml)ratio was 1:3,and phosphoric acid buffer solution's pH was 7.8,20min's extraction times,and when chloroform-ethyl alcohol mixture was 0.25 times of the extracting,and cetone is 1.5 times of the thick enzyme fluid,the separation result was better than any other conditions.This study supplied new enzyme source for superoxide dismutase.The facts indicated that the enzyme is Mn-SOD.The enzymatic activity is stable within the temperature 30-60 ℃and pH 4-8.This study supplied new enzyme resource for superoxide dismutase from newhall orange.
【Fund】: 安徽师范大学青年基金资助课题(2006xqn73)
【CateGory Index】: TQ464.8
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