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《Journal of Anhui Normal University(Natural Science)》 2010-01
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Influences of Copper and Oxalate on the Production of Ligninolytic Enzyme System of Ganoderma lucidum Under Solid-state Co-Fermentation in Cole Stalk Medium

ZHANG Ding-qian,KE Li-xia,ZHAO Wen-ke,WU Yi-xiang(College of Life Science,Anhui Normal University;Provincial Key Laboratories of Biotic Environment and Ecological Safety in Anhui,Wuhu 241000,China)  
Effects of copper at 1,1.5,2 and 2.5mmol/L and oxalate at 2,4 and 6mmol/L on the production of ligninolytic enzyme system under solid-state co-fermentation of Ganoderma lucidum and Candida utilis in cole stalk medium were studied.The results showed that 2 mmol/L copper increased significantly the activitives of laccase and the activitives of manganese peroxidase,compared to the control,the activitives were 1032.77 and 840 U/L respectively,which were 2.53 and 2.40 times compared to the control team.Compared to the control,1mmol/L copper increased significantly the activitives of lignin peroxidase,the activitives of lignin peroxidase was 319.89U/L,which was2.08 times compared to the control team,but 1.5,2 and 2.5mmol/L copper decreased significantly the activitives of lignin peroxidase,the inhibitory rates were 30.71%,18.95% and 11.11%,respectively.Compared to the control,2,4mmol/L oxalate increased significantly the activitives of laccase,the activitives of laccase were 1111.44,1301.89U/L,which were 2.73,3.2 times compared to the control team,respectively.4 mmol/L oxalate increased significantly the activitives of manganese peroxidase,the activitives of manganese peroxidase was 693.33U/L,which was 1.98 times compared to the control team,but 2,4 and 6mmol/L oxalate decreased the activitives of lignin peroxidase,the inhibitory rates were 43.03%,28.81% and 33.73% respectively.
【Fund】: 安徽省教育厅自然基金重点项目(kj2008A135);; 安徽师范大学博士科研启动基金;; 芜湖市2009年度重点科技项目(农业11-1)
【CateGory Index】: S816
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