Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Anhui Normal University(Natural Science)》 2011-06
Add to Favorite Get Latest Update

Effects of Lead on Testicular Cells Apoptosis and Expression of Caspase-3,Bc-2 and Bax Genes in Mouse

LI Jin,CAO Feng(College of Life Sciences,Anhui Normal University,Wuhu 241000,China)  
To investigate the occurrence of testicular cell apoptosis and the expression of the apoptosis related gene Caspase-3,Bcl-2 and Bax in the adult male mice following lead administration,twenty five adult mice were randomly divided into blank control group(0h) and 12h,24h,36h,48h lead exposed group(five mice in each group) and treated with lead acetate solution(100mg/kg) by intraperitoneal injection.The mice were sacrificed at 0h,12h,24h,36h and 48h respectively and testes were isolated.The testes were then fixed and processed for hematoxylin-eosin and immunohistochemical staining to detect cell apoptosis and the level of Caspase-3,Bcl-2 and Bax gene expression.Compared with the control group,the protein level of Bcl-2 was significantly lower(P0.01),whereas the protein levels of Caspase-3 and Bax were significantly higher(P0.01) in experimental groups.The degree of differences was correlated with the time of lead exposure.The testicular apoptotic cell death is highly associated with lead loading and changes in Caspase-3 and Bcl-2/Bax expression may play an important role in this process.
【CateGory Index】: X174
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 GENG Xue-Xia① DAI Xin① CHAO Qiu-Jie① YANG Zhen② ZHANG Hai-Jun① ① School of Life Sciences,Huaibei Normal University,Huaibei 235000;② No.3 High School of Guoyang County,Guoyang 233600,China;Injury of Liver and Kidney Caused by Acute Lead Poisoning and Related Molecular Mechanisms in Mice[J];Chinese Journal of Zoology;2013-04
Chinese Journal Full-text Database 4 Hits
1 YU Gao-yan~1 YAN Chong-huai~(1*) YU Xiao-gang~1 ZUO Yong~2 YU Xiao-dan~1 XU Jian~1 ZHANG Yan-ping~1 ZOU Xiang-yu~1 (1.Shanghai XinHua Hospital Affiliated to School of Medicine,Shanghai Jiao Tong University,Shanghai Institute for Pediatric Research,Shanghai Key Laboratory of Children's Environmental Health,Shanghai 200092,China;2.Dept.of Cell Biology,School of Medicine,Shanghai Jiao Tong University,Shanghai 200025,China);The Effect of Low-Level Lead Exposure on the Testicle Tissue and Hormone of Male Rabbits in Juvenile Stage[J];Journal of Environmental & Occupational Medicine;2007-03
2 Duo XU 1, Da-Nian QIN 1, Yong WANG 1, Chu-Xiang ZHUANG 1, Wei-Qiu LI 2 (1.Department of Physiology, 2.Department of Electron Microscopy, Medical College of Shantou University, Shantou, Guangdong 515031, China);Effect of Local Testicular Heating on Spermatogenic Cell Apoptosis in Rat[J];Acto andrologica Sinica;2003-03
3 ZHANG Yan,WANG Chunhong,ZHANG Duanlian,et al.School of Public Health,Wuhan University(Wuhan 430071,China);Effects of lead on expressions of TGFβ_1 and Caspase-3 in mice testes[J];Chinese Journal of Public Health;2006-07
4 Liang Cuiling,Wu Junqing,Gao Ersheng,et al.,Shanghai Institute of Planned Parenthood Research, National Laboratory of Contraceptives and Devices Research (200032),Shanghai;The Canonical Correlation Analysis on the Semen Quality and Serum Heavy Metal among Chinese Young Men[J];Chinese Journal of Health Statistics;2003-03
Chinese Journal Full-text Database 10 Hits
1 HONG Yansong~1 LI Xiumei~1 MENG He~2 ZHAN Desong~(1*) AI Hongjun~1 1.School of Stomatology.China Medical University.Shenyang 110002 2.School of Stomatology.Hebei United University.Tangshan 063000;Compare of Biocompatibility of the Different Dental Alloys[J];Chinese Journal of Materials Research;2012-02
2 Jin Longjin+1 Dong Jieying+1 Lou Zhefeng+1Chen Xiwen+2 Wang Yilong+2(1. Central Laboratory of Biology, Wenzhou Medical Collage, Wenzhou Zhejiang 325027; 2. Laboratory Animal Center, Wenzhou Medical Collage, Wenzhou Zhejiang 325027);Effects of mercuric chloride on DNA damage ofspermatogenic cells of mice testicle[J];Environmental Pollution & Control;2004-06
3 KOU Su-ru,DUAN Fei,MA You-min(Hebei Medical College for Continuing Education,Hebei Baoding 071000,China);Effects of infrared radiation on the testicles in rats[J];Academic Journal of PLA Postgraduate Medical School;2006-01
4 ZHANG Yi-min1,CHEN Shao-bing1,TANG Chun-zhi2,CAO Yong1,MA Min1(1 Chinese Medicine Department,Medical School,Jinan University,Guangdong 510632;2 School of Acupuncture and Massage,Guangzhou University of Chinese Medicine);Effects of acupuncture on expressions of brain P~(53),Bcl-2 and Bax in rats with brain contusion[J];Journal of Beijing University of Traditional Chinese Medicine;2009-06
5 LIU Feng, TAO Fang-biao, YAN Shuang-qin (1.Department of Maternal and Child health, School of Public Health, Anhui Medical University, Hefei 230032, Anhui, China; 2.Maanshan Municipal Health Hospital For Women and Children, Maanshan 243001, Anhui, China);Parental environmental exposure and occurrence of congenital heart disease in their children[J];Journal of Clinical Pediatrics;2009-05
6 LI Guang-wei,DU Guang-hui,YANG Wei-min,et al.(Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China);Experimental study of lead-induced apoptosis of spermatogenic cell in the rats[J];Journal of Clinical Surgery;2005-06
7 ZHANG Ji-qiang, QIN Da-nian, CUI Hai-yan Faculty of Physiology, Medical College of Shantou University, Shantou, Guangdong 515031, China;Relationship Between Germ Cell Apoptosis and Sertoli Cell Vimentin in Prepubertal Rats Induced by Local Exposure to Heat[J];National Journal of Andrology;2006-03
8 LIAO Wei-gong,GAO Yu-qi,CAI Ming-chun,WU Yi,HUANG Jian,FAN You-mingDepartment of Pathophysiology and High Altitude Physiology,Third Military Medical University,Chongqing,400038,China;Hypoxia Promotes Apoptosis of Germ Cells in Rat Testes[J];National Journal of Andrology;2007-06
9 Cui Haiyan, Qin Danian, Zhang Jiqiang Department of Physiology, Medical College of Shantou University, Shantou 515031,China Huang Guang Department of Paediatrics, the First Affiliated Hospital, Medical College of Shantou University;The effect of testicular local heating on spermatogenic cell apoptosis in immature rat[J];Chinese Journal of Andrology;2006-01
10 LI Jing-ping1,GUO Wen-bin2,HE Jin-can2,YU Qing-feng2, WU Xiao-qiang2,MAO Xiang-ming2,ZHANG Feng-bin1 1.Department of Reproductive Endocrinology,Women's Hospital,Zhejiang University School of Medicine,Hangzhou,Zhejiang 310006,China;2.Department of Urology,Nanfang Hospital,Southern Medical University,Guangzhou,Guangdong 510515,China;Establishing a mouse model of Sertoli-cell-only syndrome by administration of busulfan[J];National Journal of Andrology;2013-04
Chinese Journal Full-text Database 4 Hits
1 LIAN Lingjun1, WUChen1, XUJin1, WANG Xiaofeng1, XULihong1, 2, *1.Institute of Occupational and Environmental Health, Zhejiang University, Hangzhou 3100312.Department of Biochemistry and Molecular Biology, School of Medicine, Zhejiang University, Hangzhou 310031;Exposure to lead in mice leading to DNA damage and oxidative damage[J];Acta Scientiae Circumstantiae;2006-01
2 WU Chen 1 , LIAN Lingjun 1 , XU Jin 1 , WANG Xiumin 2 , XU Lihong 2, * 1. Institute of Occupational and Environmental Health , Zhejiang University , Hangzhou 310031 2. Department of Biochemistry and Molecular Biology , College of Medicine , Zhejiang University , Hangzhou 310031;Effects of lead on the ultrastructure and expression of p53、Bax and Bcl-2 in mice liver[J];Acta Scientiae Circumstantiae;2006-09
3 ZHAO Jian1,TONG Xi-qiong2,CAI Ya-fei 1(1.College of Life Science,Anhui Normal University,The Key Laboratory of Bioresource Protection and utilization of Anhui province The Key laboratory of Biotic Environment and Ecological Safety in Anhui Province;2.University Hospital of Anhui Normal University,Wuhu 241000,China);The preliminary researches on the toxic effects of Pb on mouse blood,liver and kidney[J];Journal of Biology;2010-01
4 JIA Qing-hua,JIANG Jun-jun,HA Xiao-qin;Effect of lead acetate on apoptosis astrocytes and Caspase-3 expression in mice[J];China Occupational Medicine;2010-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Ruian △, ** Takehiko Koji, *Zheng Pingju, Zhang Yuanqiang, ** Paul K. Nakane (Department of Histology and Embryology,Institute of Basic Medicine; Department of Oncology,Xijing Hospital,The Fourth Military Medical;PROGRAMMED CELL DEATH OF MALE GERM CELLS OF MOUSE DURING POSTNATAL DEVELOPMENT[J];ACTA ANATOMICA SINICA;1998-04
2 Jian-xing Xu(National Laboratory of Biomacromolecule, Institute of Biophysics,CAS, 100101 Beijing);The Antioxidative Function of Cytochrome C in Mitochondria[J];Bulletin of the Chinese Academy of Sciences;2003-04
3 Yan Chonghuai,Shen Xiaoming,Xue Minbo,Yu Xiaogang,Jin Xingming,Zhang Yanping,Markowitz M.Xinhua Hospiˉtal,Shanghai Children's Medical Center affiliated to Shanghai Second Medical University,Shanghai Institute for Pediatric Research,Shanghai200092;A clinical analyses of102patients with childhood lead poisoning in Shanghai[J];The Journal of clinical Pediatrics;2003-04
4 Hong Lin YIN 1, Xiao Jun ZHOU 1, Yu Feng HUANG 2, Ying Xia CUI 2, Zhen Feng LU 1. (1. Department of Pathology; 2. Centre of Medical Laboratory Science, Nanjing General Hospital of Nanjing Command,PLA,Nanjing,Jiangsu 210002,China);Ultrastructural Features of Human Apoptotic Spermatogenic Cells[J];Acto andrologica Sinica;2001-01
5 DuoXU ,Da NianQIN (DepartmentofPhysiology ,MedicalCollegeofShantouUniversity , Shantou ,Guangdong 5 15 0 31,China);Vasectomy Induce Testicular Germ Cell Apoptosis[J];Acto andrologica Sinica;2001-06
6 XU Jian-Xing ** (National Laboratory of Biomacromolecules,Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China);The Role of Electron Leakage of Mitochondrial Respiratory Chain in Cell Apoptosis[J];Progress In Biochemistry and Biophysics;2003-04
7 LUO Chun, ZHU Cheng, LU Yicheng, et al. Department of Neurosurgery, Changzheng Hospital, the Second Military Medical University, Shanghai 200003;Spatial and temporal profile of apoptosis following lateral fluid percussion brain injury in rat[J];Shanghai Medical Journal;2002-02
8 Lei Duan,Wei Ping Wang,Jian Shen Zhang,Yong Zhong Ding,Department of Neurosurgery, Second Affiliated Hospital of Lanzhou Medical College, Lanzhou 730030,Gansu Province, Gansu;Nerve cell apoptosis and expression of Bc1-2/Bax after cerebral contusion/laceration in human beings[J];Modern Rehabilitation;2003-16
9 XU Jian,Yan Chonghuai,YU Xiaogang,et al.Department of Environmental Health,Shanghai Second Medical University Affiliated Xinhua Hospital,Institute of Shanghai Pediatric Research(Shanghai 200092,China);Effect of lead on cyclic AMP level in cultured hippocampal neuron[J];China Public Health;2005-03
10 YANG Xinyu, YANG Shuyuan, ZHANG Jianning, et al. General Hospital of Tianjin Medical University, Tianjin 300052,China;An experimental observation on delayed neuronal death after acute brain trauma[J];Chinese Journal of Traumatology;2001-09
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved