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《Journal of Anhui Traditional Chinese Medical College》 2001-01
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QUANTITATIVE DETERMINATION OF BAICALIN IN XIAOCHAIHU GRANULE BY RP-HPLC

SHEN Jing,LIU Feng,ZHANG Chun-lai,ZHAO Xiang-yang (Lianyungang Kanion Pharmaceutical Co.Ltd,Jiangsu Lianyungang 222001,China)  
Objective:Quantitative determination of Baicalin in Xiaochaihu Granule by RP-HPLC.Method:Baicalin was extracted with methanol by an ultrasonic wave.Chromatographic conditions were as follows:Alltima C 18 column(250mm×4.6mm,5μm);Mobile phase:methanol-water-phosphoric acid(60∶40∶0.2);Column tempareture:the temperature of laboratory;Detection wavelength:280nm.Result:The standard curve was linear in the range of 0.06~0.60μg.The average recovery rate and RSD were 100.23% and 1.70% respeclively.(n=5).Conclusion:This method is rapid,reliable.It is suitable for quality control of the production.
【CateGory Index】: R284.1
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