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《Journal of Anhui University of Chinese Medicine》 2017-04
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Differentially Expressed miRNAs in Rat Osteoblasts Treated by Serum Containing Bushen Prescription

LIANG Xing-lun;PAN Xin;ZENG Si-liang;JI Cheng-dong;ZHOU Wen-rui;LI Chen;ZHU Min-jie;LIU Chuan-song;ZHOU Wen-bo;XU Cheng;QIAN Shu-wen;YU De-hua;ZHANG Shao-wei;ZHU Wei-hong;Yangpu Hospital Affiliated to Tongji University;Dinghai Community Health Service Center;  
Objective To investigate the differentially expressed miRNAs in rat osteoblasts treated by serum containing Bushen Prescription,the target miRNAs for the treatment of osteoporosis with Bushen Prescription,and mechanisms for the therapeutic effect of Bushen Prescription by regulating the expression of target genes.Methods Primary rat osteoblasts were treated by serum containing Bushen Prescription after identification.The miRNA microarray was used to measure the expression of miRNAs in osteoblasts treated by serum containing Bushen Prescription for 1or 3days,and real-time quantitative PCR was used to further validate the miRNAs which were2-fold differentially expressed between the two groups.Results The primary culturedosteoblasts had the typical properties of osteoblasts.A total of 24 miRNAs were2-fold differentially expressed between the two groups,and compared with the control serum group,the serum containing Bushen Prescription had 16up-regulated miRNAs and 8down-regulated miRNAs.The real-time PCR results for these 24 miRNAs were consistent with the microarray results.Conclusion Bushen Prescription can regulate target genes via differentially expressed miRNAs in osteoblasts and thus improve osteoporosis.
【Fund】: 国家自然科学基金项目(81173312 30972633);; 国家重点基础研究发展计划(2013CB531601);; 上海市卫生和计划生育委员会科研项目(201640253)
【CateGory Index】: R285.5
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