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《Modern Agricultural Science and Technology》 2019-02
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Cloning and Bioinformatic Analysis of Key Enzyme in Camptotheca acuminata

WANG Yao-yao;DING Jian-lan;HAN Zhuo;GUO Ya-xin;LIU Shi-hui;LIU Xiao-ning;School of Medicine,Huanghe S&T University;  
In order to clone the STR gene of strictosidine synthase,a key enzyme in the biosynthesis pathway of Camptotheca acuminata,conduct bioinformatics analysis on it,and reveal the basic physical and chemical properties of the protein,RNA extraction was conducted on young leaves of Camptotheca acuminata,and CDS sequences of the STR gene were cloned by RT-PCR.ExPASy ProtParam,SignalP 4.1,TMHMM v. 2.0 and TargetP1.1 were used to analyze the physical and chemical properties,protein signal peptide,protein transmembrane region and subcellular localization of proteins.Conserved Domain and SOPMA were used to predict the conserved domain and secondary structure of proteins, and MEGA 6.0 software was used to construct the protein phylogenetic tree.The results showed that, using bud of Camptotheca acuminataas material, CDS sequence of STR gene was 993 bp by RT-PCR.Bioinformatics analysis showed that,STR gene located in the secretory channel,which was an acidic protein with no signal peptide,and had a transmembrane region consisting of secondary structures such as α-helix,extended strand,β-turn,and random coil. STR gene contained highly conserved domains, and cluster analysis indicated that it had high similarity, close genetic relationship and genetic distance with Coffea canephora.
【Fund】: 河南省大学生创新创业训练项目(201711834028)
【CateGory Index】: Q943.2;S792.99
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【Citations】
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1 MA Lei1,ZHANG Xiang2,TIAN Yongqiang1,ZHANG Guolin2& LUO Yinggang2(1College of Chemical Engineering,Sichuan University,Chengdu,610065,China)(2Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China);Molecular Cloning and Heterologous Expression of Putative Strictosidine Synthases from Arabidopsis thaliana[J];应用与环境生物学报;2013-02
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