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《Journal of Anshan Normal University》 2010-02
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Soluble Expression and Purification of Recombinant Human Cyclin D1 in E.coli BL21

ZOU De-sheng ZHANG Bo ZHANG Lan-jie XIN Guang LI Gui-ying(Department of Chemistry,Anshan Normal University,Anshan Liaoning 114007,China)  
The purpose of the study is to obtain soluble expression protein cyclin D1.The recombinant vector pET-20b-cycD is constructed by inserting human cyclin D1 gene into pET-20b plasmid and is identified by digestion with restriction enzymes.An expression strain is selected after transformation of recombined plasmid into E.coli BL21.Part of recombinant protein secrete into culture medium after induced by IPTG.Recombinant protein is purifed by Ni2+ chelate chromatography and the purity of purified protein is up to 95%.A specialized band can be see in molecular weight of about 33 000 after SDS-PAGE(SDS-polyacrylamide gel electrophoresis) analysis and Western blotting with an anti-cyclin D1 antibody.The recombinant cyclin D1 is successfully soluble expressed and purified.
【CateGory Index】: Q78
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHOU Xiao-jian, ZHANG Ping, PAN Hong-ya, CHEN Wan-tao. (Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, School of Stomatology, Shanghai Second Medical University. Shanghai 200011, China);The influence on expression of cyclin D1 by anti-sense oligonucleotide expression vector[J];Shanghai Journal of Stomatology;2005-02
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 ZHOU Xiao-jian, YU Yang, PAN Hong-ya, CHEN Wan-tao,ZHANG Ping,ZHANG Zhi-yuan. Department of Oral and Maxillofacial Surgery,School of Stomatology; Ninth People′s Hospital,School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China;Inhibition effect on Tca8113/CDDP by antisense oligonucleotides of cyclin D1 combined with cis diamminedichloroplatinum[J];Chinese Journal of Stomatology;2006-06
2 ZHOU Xiao-jian,ZHANG Zhi-yuan,ZHANG Ping,PAN Hong-ya,CHEN Wan-tao, YE Dong-xia .Department of Oral and Maxillofacial Surgery, School of Stomatology;Ninth People′s Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200011, China;In vitro gene silencing by siRNA of cyclin D1 on tongue squamous cell carcinoma cell line resistant to cisplatin[J];Chinese Journal of Stomatology;2006-11
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHOU Xiao-jian,LI Qin,CHEN Wan-tao.(Department of Oral Maxillofacial Surgery,School of Stomatology,Ninth People's Hospital,Shanghai Second Medical University,Shanghai200011,China);Establishment and Drug-Resistance Gene Expression of Cisplatin-Resistant Cell Line Tca8113/CDDP[J];Shanghai Journal of Stomatology;2003-06
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