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《Journal of Anshan Normal University》 2018-02
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Determination of total steroids in mushrooms

GUO Hua;DIAO Quanping;SUN Tingting;HOU Dongyan;School of Chemistry and Life Science,Anshan Normal University;  
In the study,a method for determining of total steroids in mushrooms was developed.The total steroids were determined by UV spectrophotometry with ergosterol as reference substance after Liebermann-Burchard reaction.The reference substance and samples were heated at 40 ℃ for 20 min after adding in chromogenic agents,then cooled to room temperature,the chromogenic reaction was stable from 20 min to 25 min. The detection wavelength was 670 nm.The linear concentration range of total steroids was( 0.04 ~ 0.24) mg/m L( r =0.999 1) and the average recovery was 101.78% with RSD of 1.79%.The method is simple and accurate,and can be used for the determination of total steroids in mushrooms.
【Fund】: 辽宁省自然科学基金资助课题(2015020680)
【CateGory Index】: R284.1
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