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《Oncology Progress》 2018-02
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Effects of Numb protein and Numb/Notch signaling pathway on radiosensitivity of human pancreatic cancer cell line

LU Xiaoyu;Department of Pathology, He'nan Cancer Hospital;  
Objective To investigate the effect of Numb protein and Numb/Notch signaling pathway on the radiosensitivity of human pancreatic cancer cell lines. Method Human pancreatic cancer MIA PaCa-2 were treated with radiation. The cell viability of each group was detected by MTT, the total apoptosis rate was detected by flow cytometry. Cell scratch assay and Transwell assay were used to determine cell migration and invasion. Western blot was used to detect the mRNA and Numb proteins of Numb/Notch signaling pathway and downstream molecules of Notch1, Hes1 and Hes5. Re Result MTT assay showed that with the increase of radiation dose, the absorbance values of MIA PaCa-2 cells were lower than those of non-irradiated cells(P0.05). Cell apoptosis assay revealed that with the increase of radiation dose, the overall apoptosis rate of MIA PaCa-2 cells was higher than that of non-irradiated cells(P0.05). Cell scratch assay and Transwell assay demonstrated that with the increase of radiation dose, the migration and invasion ability of MIA PaCa-2 cells were weakened, the invasion and migration ability at the radiation dose of 1, 3, and 5 Gy were lower than that of non-irradiated cells(P0.05). qRT-PCR and Western blot cells indicated that as the radiation dose increased, the expression of Numb increased obviously, expressions of mRNA and protein in Notch1, Hes1 and Hes5 were lower than that in non-irradiated cells(P0.05). Conclusion The expression of Numb protein increased, and the Numb/Notch signaling pathway was activated in pancreatic cancer cells. The Numb/Notch signaling pathway was related to the radiation dose of X ray in pancreatic cancer cells.
【CateGory Index】: R735.9
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