Effect and mechanism of proanthocyanidins on proliferation and glycolysis of esophageal cancer cells
WANG Jungang;YANG Li;TIAN Junna;ZHANG Jie;Department of Thoracic Surgery, Nanyang Central Hospital;
Objective To investigate the effect and mechanism of proanthocyanidins on the proliferation and glycolysis of esophageal cancer cells. Method Esophageal cancer cells OE33, CP-C and Eca109 were treated with 0, 60, 120,180, 240 and 300 μmol/L proanthocyanidins respectively, and the cell survival was detected with MTT assay for calculating half maximal inhibitory concentration(IC_(50)), which was subsequently used to treat Eca109 cells, and then the ATP content, pyruvate kinase(PK) activity, hexokinase(HK) activity and lactic acid content in the supernatant were detected using testing kits, the expression levels of Akt, p-Akt, STAT3 and p-STAT3 were detected by Western blot. Result Proanthocyanidins can inhibit the proliferation of esophageal cancer cells OE33, CP-C and Eca109 in a concentration-dependent manner, the IC_(50) was(285.48±3.58)μmol/L,(291.00±3.36)μmol/L,(237.95±4.91) μmol/L, respectively, and the last concentration demonstrated the maximum inhibitory effect on Eca109 cell line cells, and were selected as the study concentration. After treatment with 240 μmol/L proanthocyanidins on Eca109 cell line cells, the ATP content, PK activity,HK activity and secretion of lactic acid levels were significantly reduced, and the expression levels of p-Akt and p-STAT3 also declined obviously as compared with the treatment group of 0 μmol/L(P0.05). Conclusion Proanthocyanidins can inhibit the proliferation of esophageal cancer cells, influencing the glycolytic pathway of esophageal cancer cells, of which the mechanism may be involved with Akt and STAT3 signaling pathways.
【CateGory Index】： R735.1