Effect of pantoprazole on proliferation and apoptosis of gastric cancer cell MKN-45
WANG Fengji;LU Yanjun;CHENG Xiangqin;Department of General Surgery, Shandong Institute of Parasitic Diseases (Shandong Control Center for Digestive Diseases);
Objective To explore the effect of pantoprazole on the proliferation and apoptosis in gastric cancer cell MKN-45 and the underlying mechanism. Method MKN-45 cell was treated with 0, 10, 20, and 40 μg/ml pantoprazole for 48 h; cell viability was measured by MTT assay; cell morphology was detected by Hoechst staining; cell cycle was determined by flow cytometry; the expression of B-cell lymphoma-2(Bcl-2), Bcl-2 associated X protein(Bax), Cyclin D1 and Cyclin E were measured by western blot. Result Compared with 0 μg/ml, treatment of 10, 20, 40 μg/ml pantoprazole reduced cell viability(P0.01), resulting in dense and thick white cell nucleus, with cell cycle arrested in G1 phase(P0.01), besides, the expression of Cyclin D1 and Cyclin E was down-regulated(P0.01). 20 and 40 μg/ml pantoprazole might markedly down regulate the expression of Bcl-2(P0.01), while up regulate the expression of Bax(P0.01). Con Conclusion Pantoprazole could inhibit MKN-45 cell proliferation and induce cell apoptosis, by regulating expression of cell cyclin and apoptosis related protein.
【CateGory Index】： R735.2